一株异养硝化菌AD-28氨氮去除特性研究

以异养硝化菌——嗜碱假单胞菌AD-28为试验对象,通过单因子试验测定好氧条件下碳源、碳氮比、温度、pH、溶解氧、初始氮质量浓度及盐度等不同因素对嗜碱假单胞菌AD-28生长及氨氮去除的影响。试验结果表明,适用于嗜碱假单胞菌AD-28生长和氨氮去除的最佳条件为:碳源柠檬酸钠、丁二酸钠、乙酸钠,碳氮比20,温度25～35℃,pH 6.0,转速120r/min;在最佳条件下,嗜碱假单胞菌AD-28对初始质量浓度为20～160mg/L的氨氮去除率在24h内达95%以上。嗜碱假单胞菌AD-28能直接以氨氮为底物进行高效的硝化作用,并能耐受较低的温度(15℃)和较高的盐度(NaCl质量浓度为50g/L),在调节养殖水体水质方面具有广阔的应用前景。

Ammonia Nitrogen Removal Characteristics of a Heterotrophic Nitrifier AD-28

The effects of carbon source,C/N ratio,temperature,pH,dissolved oxygen,salinity and initial ammonia nitrogen concentration on growth and ammonia nitrogen removal efficiency of Pseudomonas alcaliphila AD-28 as a high efficiency heterogeneous nitrifier were studied under the aerobic conditions in a single-factor experiment.The results showed that the optimum growth and nitrification was observed in AD-28 under conditions of sodium citrate,sodium acetate and sodium succinate as carbon resources,C/N ratio of 20,25-35℃,pH 6.0,and a rotating speed of 120 r/min,with ammonia nitrogen removal rate of up to 95%treated by AD-28 for 24 h at initial ammonia nitrogen concentration from 20 mg/L to 160 mg/L.The findings indicated that strain AD-28 exhibited an amazing ability to remove the ammonia nitrogen at low temperature(15℃)and high salt tolerance(NaCl concentration of 50 g/L),which has great potential treatment of aquaculture water.