硫酸铜对双斑东方鲀刺激隐核虫病的治疗效果研究

刺激隐核虫病是双斑东方鲀养殖生产中的主要致死性寄生虫病,为安全、有效地防治刺激隐核虫病,开展了硫酸铜对双斑东方鲀幼鱼的急性毒性试验及硫酸铜对双斑东方鲀刺激隐核虫病的治疗效果试验。急性毒性试验结果显示,硫酸铜对双斑东方鲀幼鱼24、48、72、96 h半致死质量浓度分别为7.94、7.12、5.32、4.44 mg/L,其安全质量浓度为1.72 mg/L。刺激隐核虫病治疗试验结果表明,1.0、1.5 mg/L的硫酸铜质量浓度对病鱼治疗有效,药浴后刺激隐核虫虫体活力下降,第3 d部分虫体解体或脱落,药浴5 d治愈;0.5 mg/L硫酸铜对病鱼治疗效果不佳,病情继续发展,第8 d病鱼死亡。临床上建议使用硫酸铜质量浓度1.0 mg/L治疗双斑东方鲀刺激隐核虫病。本研究对其他鱼类刺激隐核虫病及其纤毛虫病治疗也具有一定的借鉴意义。

Therapeutic Effect of Copper Sulfate on Cryptocaryoniosis in Twospot Puffer Takifugu bimaculatus

The acute toxicity of copper sulfate to twospot puffer Takifugu bimaculatus with total length of (3.51±0.20) cm and body weight of (1.18±0.26) g was studied by 96 h semi-static bioassays under conditions of water temperature of (27±1)℃, salinity of 23-25, pH of 7.90-8.10 and dissolved oxygen level of over 6.00 mg/L. The lethal concentration 50 (LC 50 ) of copper sulfate to twospot puffer was found to be 7.94 mg/L for 24, 7.12 mg/L for 48 h, 5.32 mg/L for 72 h, and 4.44 mg/L for 96 h, with safe concentration of 1.72 mg/L. The therapeutic effect of copper sulfate on Cryptocaryon irritans infected fish with total length of (8.18±0.91) cm and body weight of (13.08±4.44) g was studied in sand filtered seawater at water temperature of 22-24 ℃, a salinity of 23-25, pH of 7.90-8.10 and dissolved oxygen level of over 6.50 mg/L. The protozoon Cryptocaryon irritans had loss of vitality in infected twospot puffer bathed in copper sulfate solution at concentrations of 1.0 mg/L and 1.5 mg/L, disintegration or abscission of worm at 3rd day and cured at 5th day after treatment. However, the diseased fish continued to develop and died on the 8th day at copper sulfate concentration of 0.5 mg/L. It is recommended that 1.0 mg/L of copper sulfate be used as the clinical treatment concentration for cryptocaryoniosis therapy.