| 武夷岩茶种质资源遗传多样性与亲缘关系的SRAP分析 |
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| 引用本文： | 夏法刚,衷兴旺,吴锋,季彪俊.武夷岩茶种质资源遗传多样性与亲缘关系的SRAP分析[J].茶叶科学,2017,37(1). |
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| 作者姓名： | 夏法刚 衷兴旺 吴锋 季彪俊 |
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| 作者单位： | 1. 福建农林大学作物科学学院,福建 福州,350002;2. 武夷山市茶叶产品质量检验所,福建 武夷山,354300 |
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| 基金项目： | 国家质检总局科技计划项目 |
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| 摘 要： | 本研究利用SRAP-PCR反应体系,从88对SRAP引物组合中筛选出43对引物组合,对武夷岩茶主要的30份种质资源进行SRAP分析,共扩增出385条带,其中具有多态性的有236条带,占总数的61.30%,表明30份武夷岩茶种质呈现一定的遗传多样性。武夷岩茶种质资源间遗传距离变幅在0.31~0.98之间,平均为0.63,基于SRAP标记利用系统聚类法把武夷岩茶分为三大类,为武夷岩茶遗传研究、品种选育与资源保护提供参考。
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| 关 键 词： | 武夷岩茶 种质资源 SRAP标记 遗传多样性 亲缘关系 |
SRAP Marker Analysis of Genetic Diversity and Relationship in Wuyi Rock Tea Germplasm Resources |
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| XIA Fagang,ZHONG Xingwang,WU Feng,JI Biaojun.SRAP Marker Analysis of Genetic Diversity and Relationship in Wuyi Rock Tea Germplasm Resources[J].Journal of Tea Science,2017,37(1). |
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| Authors: | XIA Fagang ZHONG Xingwang WU Feng JI Biaojun |
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| Abstract: | The genetic diversity and relationship of 30 Wuyi rock tea germplasms were analyzed by Sequence-related amplified polymorphism (SRAP) markers. Forty three pairs of primers selected from 88 pairs of candidate SRAP primers produced 385 amplified fragments. Among them, 236 were polymorphic, accounting for 61.30% of the total fragments. The results showed that there was distinct genetic diversity within Wuyi rock tea germplasms. The genetic distance ranged from 0.31 to 0.98 with an average of 0.63. Thirty Wuyi rock tea germplasms were classified as three categories basing on the UPGMA cluster analysis. This study was of great significance for genetic research, breeding and resource protection of Wuyi rock tea. |
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| Keywords: | Wuyi rock tea germplasms SRAP marker genetic diversity genetic relationship |
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