基于阵列芯片现场高灵敏同步快速检测粮油中氨基甲酸酯类农药残留混合污染

On-site rapid sensitive and simultaneous detection for multiple carbamate pesticide residues in grains and oils based on microarray

The quality and safety of agricultural products is the technique focus of the strategy of rural vitaliza?tion. Carbamate pesticide residues are seriously threatening the safety of agricultural product and human life and health. Nowadays, the common detection methods are instrument-based for carbamate pesticide residue, which dis? satisfy the request of an on-site rapid, sensitive and simultaneous detection for multiple carbamate pesticide resi? dues. Based on the home-made monoclonal antibodies against carbofuran and carbaryl respectivity, a microarray for rapid and simultaneous detection of multiple carbamate pesticide residues was developed within 30 min. Under the optimized conditions, the limits of detection（LODs）were found to be 0.11 and 0.09 ng/g, while the linear ranges were 0.33-3000 and 0.18-1500 ng/g for carbofuran and carbaryl respectively. By using spiked peanut samples, it recorded recoveries of 88.4%-98.3%, 90.1%-118.8% for carbofuran and carbaryl respectively. The RSD of intraarray were found at 9.0%, 3.5%, while the RSD of inter-array were recorded at 12.3%, 16.3% respectively. The de? tection results of the microarray were highly consistent with that of HPLC-MS/MS. This proposed microarray provid? ed an on-site high-sensitivity, simultaneous and rapid detection of carbofuran and carbaryl, and could be widely used in the fields of agricultural product quality safety, food safety and environmental monitoring.