不同状态培养基下pH对香菇、滑菇及金针菇漆酶活性的影响

考察不同物理状态培养基条件下pH对香菇、滑菇、金针菇漆酶活性的影响,为香菇、滑菇、金针菇的栽培提供重要参考。固化培养基以及液体培养基以NaOH作为碱化剂,固体培养基以壳灰作为碱化剂,考察不同pH对3种食用菌漆酶活性的影响。结果表明：微碱化培养可提高3种不同食用菌的漆酶活力,并与培养基的物理状态有关。香菇在固化、液体、固体培养基中,pH分别为6.5、6.5以及6.0时漆酶活性最高,比对照提高5.3%~14.0%;金针菇适宜的pH分别为7.0、6.0和6.5,与对照相比提高35.9%~343.0%;滑菇适宜的pH均为6.0,与对照相比提高3.1%~94.9%。

Effects of pH on Laccase Activity of Lentinula edodes,Pholiota nameko,and Flammulina velutipes Grown in Different State Media

Laccase is a key enzyme involved in the degradation and utilization of lignin and has a relationship with mushroom cultivation cycle and product quality. In this work, NaOH was used as the alkalization agent for both solidified media and liquid media, and shell ash was used for solid media, the effect of pH on the activity of laccase of Lentinula edodes, Pholiota nameko, and Flammulina velutipes grown in different state media was investigated for providing a basis for their cultivation. Results showed that the micro-alkalization of all tested culture media could improve the activity of laccase of the species, and its effect was related to media type. The optimal pH values of solidified, liquid, and solid media for L. edodes laccase activity was 6.5, 6.5 and 6.0, respectively, with a 5.3%-14.0% increase of laccase activity in relative to their controls, and for that for F. velutipes was 7.0, 6.0, and 6.5, and the increase in the activity under optimal pH values was 35.9%-343.0% compared with their controls. As to P. nameko, pH 6.0 was optimal for all three media for maximizing laccase activity, 3.1%-94.9% higher than those of their controls.