艾纳香中的黄酮类化合物及其抗菌活性

为明确艾纳香抗菌药效物质基础,采用硅胶柱色谱, 凝胶色谱和反相色谱等技术从艾纳香乙酸乙酯部位中分离获得15个单体化合物,经波谱学鉴定分别为：3,3°,5-三羟基-4°,7-二甲氧基二氢黄酮 (1)、4°,5-二羟基-3,3°,7-三甲氧基黄酮 (2)、艾纳香素 (3)、3,5,3°,4°-四羟基-7-甲氧基黄酮 (4)、香叶木素 (5)、3°,4°,5-三羟基-3,7-二甲氧基黄酮 (6)、异鼠李素 (7)、chrysosplenol C (8)、金丝桃苷 (9)、异槲皮苷 (10)、3°,5,7-三羟基-4°-甲氧基二氢黄酮 (11)、sakuranetin (12)、pilloin (13)、5,7,3°,4°-四羟基-3-甲氧基黄酮 (14)、5-羟基-3,7,3°,4°-四甲氧基黄酮 (15),其中化合物7、11、12和13为首次从该植物中分得。抗菌活性评价结果显示：化合物1、3、6、8和12对3株细菌具有不同程度的抑制活性,其中化合物3对金黄色葡萄球菌抑制活性最强, 最低抑菌浓度MIC值为32 μg/mL。

Antibacterial Activity of Flavonoids from Blumea balsamifera

To investigate the pharmacodynamic substance basis of Blumea balsamifera (L.) DC., fifteen compounds were isolated from the EtOAc fraction by silica gel, sephadex LH-20 and ODS-A column chromatography. The structures were determined based on the analysis of the spectroscopic data as 3,3°,5-trihydroxy-4°,7-dimethoxy flavanone (1), 4°,5-dihydroxy-3,3°,7-trimethoxy flavone (2), blumeatin (3), 3,5,3°,4°-tethydroxy-7-methoxy flavone (4), diosmetin (5), 3°,4°,5-trihydroxyl-3,7-dimethoxy flavone (6), isosamicin (7), chrysosplenol C (8), hyperoside (9), isoquercitrin (10), 3°,5,7-trihydroxy-4°-methoxy flavanone (11), sakuranetin (12), pilloin (13), 5,7,3°,4°-tetrahydroxy-3-methoxyflavone (14) and 5-hydroxy-3,7,3°,4°-tetramethoxy flavone (15). Compounds 7, 11, 12 and 13 are isolated from the plant for the first time. Antibacterial test indicated that 1, 3, 6, 8 and 12 displayed different inhibition against three bacteria, and 3 significantly inhibited Staphylococcus aureus subsp. aureus (DSM 799) with a MIC value of 32 μg/mL.