| 胡葱黄条病毒吉林毛葱分离物全基因组序列测定与分析 |
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| 引用本文： | 马俊丰,张宸,张春雨,李小宇,王永志.胡葱黄条病毒吉林毛葱分离物全基因组序列测定与分析[J].中国蔬菜,2020,1(6):44-48. |
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| 作者姓名： | 马俊丰 张宸 张春雨 李小宇 王永志 |
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| 作者单位： | 吉林省农业科学院植物保护研究所，吉林公主岭 136100 |
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| 基金项目： | 国家重点研发计划项目（2017YFD0201604），农业部东北作
物有害生物综合治理重点实验室开放课题（DB2018-08） |
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| 摘 要： | 使用RT-PCR方法从吉林省疑似感染胡葱黄条病毒(Shallot yellow stripe virus,SYSV)的分蘖洋葱(毛葱)叶片中获得了SYSV吉林毛葱分离物SYSV-JL(MN607702)的全基因组序列。SYSV-JL的全长序列为10?427 nt,与GenBank已登录的3条SYSV全长或近全长序列在多个基因上保持着较高的序列一致性,但在P1基因上核苷酸和氨基酸序列一致性较低,分别为69.5%～84.2%和60.9%～76.9%,进一步通过变异分析发现,P1基因包含369个变异位点,表现出较为明显的高变异特征。系统发育分析显示,不同SYSV分离物具有较为明显的地区差异,SYSV-JL分离物与多个中国分离物系统发育关系较近。
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| 关 键 词： | 胡葱黄条病毒 分蘖洋葱（毛葱） 全基因组 变异分析 |
Measuring and Analysis of Complete Genome Sequencing of Shallot yellow stripe virus Isolate from Tillering Onion in Jilin，China |
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| MA Jun-feng,ZHANG Chen,ZHANG Chun-yu,LI Xiao-yu,WANG Yong-zhi.Measuring and Analysis of Complete Genome Sequencing of Shallot yellow stripe virus Isolate from Tillering Onion in Jilin，China[J].China Vegetables,2020,1(6):44-48. |
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| Authors: | MA Jun-feng ZHANG Chen ZHANG Chun-yu LI Xiao-yu WANG Yong-zhi |
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| Affiliation: | Institute of Plant Protection，Jilin Academy of Agricultural Sciences，Gongzhuling 136100，Jilin，China |
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| Abstract: | The complete genome of Shallot yellow stripe virus（SYSV） Jilin isolate SYSV-JL（MN607702）
was cloned from SYSV infected tillering onion by RT-PCR．The complete sequence of SYSV-JL consisted of
10 427 nucleotides，and shared high sequence identity with 3 SYSV isolates that had submitted to GenBank
in many gene segments，but the sequence identity of nucleotide and amino acid in P1 gene was lower，
only sharing 69.5%-84.2% and 60.9%-76.9%，respectively．Further variation analysis showed that P1 gene
contained 369 variation sites，showing obvious high variation characteristics．Phylogenetic analysis indicated
that different SYSV isolates had distinct regional differences and the phylogenetic relationship between SYSV-JL
isolate and multiple Chinese isolates was closer． |
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| Keywords: | Shallot yellow stripe virus（SYSV） Tillering onion Complete genome Variation analysis |
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