| 培养条件对栗疫菌毒素产生的影响 |
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| 引用本文： | 韩珊,朱天辉,李芳莲.培养条件对栗疫菌毒素产生的影响[J].四川林业科技,2009,30(1):66-70. |
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| 作者姓名： | 韩珊 朱天辉 李芳莲 |
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| 作者单位： | 四川农业大学林学园艺学院,四川,雅安,625014 |
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| 基金项目： | 国家自然科技资源共享平台基金(2005DKA21207-13);;四川省学术和技术带头人培养基金资助 |
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| 摘 要： | 本文以中国板栗当年生幼嫩枝条作为栗疫菌粗毒素生物检测材料，对该病菌的产毒条件进行了研究。结果表明：栗疫菌在不同培养液的产毒能力有明显的差异，7种培养液中C（PD＋板栗煎汁）培养液产毒能力最强；栗疫病菌产毒能力以培养温度为26℃，培养基pH值为6的条件下培养18d生物活性最强；最佳氮源为蛋白胨；较好的碳源为糊精。
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| 关 键 词： | 板栗疫菌 真菌毒素 培养条件 |
The Effects of Cultural Conditions on Production of Cryphonectria parasitica Phytotoxin |
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| HAN Shan,ZHU Tian-hui,LI Fang-lian.The Effects of Cultural Conditions on Production of Cryphonectria parasitica Phytotoxin[J].Journal of Sichuan Forestry Science and Technology,2009,30(1):66-70. |
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| Authors: | HAN Shan ZHU Tian-hui LI Fang-lian |
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| Affiliation: | College of Forestry and Horticulture;Sichuan Agricultural University;Ya'an 625014 |
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| Abstract: | Young branches of Chinese chestnut were selected to test the toxicity of crude toxin by Cryphonectria parasitica, aiming to study of the conditions of phytotoxin-producity capability. The results showed that C liquid medium had the strongest phytotoxin in 7 media. The optimal cultural conditions for producing toxin were as follows ：the temperature was 26℃ ,the cultured period lasted 18 d ,and pH value was 6. Peptone was the best nitrogenous source. Dextrin was better among the selected carbonic sources. |
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| Keywords: | Cryphonectria parasitica Phytotoxin Cultural conditions |
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