禽呼肠孤病毒血清学相关性及S3基因序列同源性分析

采用血清学交叉中和试验方法,分析了4株禽呼肠孤病毒株CL(鸡源禽呼肠孤病毒)、YH和YB(鸭源禽呼肠孤病毒)及S1133(禽呼肠孤病毒标准株)之间抗原的相互关系;并对CL株的S3基因进行克隆测序,用DNAsis和Prosis等分析软件进行序列比较及推导氨基酸序列分析.结果表明:4株禽呼肠孤病毒株之间具有共同的群特异抗原,但不同病毒株间存在中和抗原位点的不对称性;血清学相关性与S3基因序列及其推导的σB蛋白氨基酸序列同源性不一致,表明禽呼肠孤病毒诱导机体产生特异性中和抗体的病毒蛋白不止σB蛋白一个,σB氨基酸序列同源性与血清学相关性没有必然的直接联系.

Serologic correlation and sequence homology of S3 gene in four avian reovirus isolates

Four strain avian reovirus,CL(isolated from chicken),YH(isolated from muscovy duck),YB(isolated from muscovy duck) and S1133(standard strains) were examined in cross-neutralization tests using corresponding antiserum,and the antigen relationship of avian reovirus was analyzed.At the same time,the S3 gene of DRV-CL was cloned and sequenced,and the amino acid sequence of polypeptide displayed on phage was deduced.Our results show that the four virus isolates share related antigenic sites.Interestingly the antigenic epitopes among the isolates exhibit unsymmetrical characteristics.There was inconsistence between serologic correlation and the sequence homology of σB protein genes.This work provides evidence for the existence of induce specific antibodies other than σB protein in avian,and the sequence homology of σB protein genes has no direct inevitable relation to serologic correlation.