| 腹泻犬源大肠埃希菌耐药性以及整合子–基因盒检测研究 |
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| 引用本文： | 魏斌,欧红萍,严光文,田一男,但佳明,涂蕊,肖启程,朱子琦,唐丽,杨亭玉,彭广能,钟志军.腹泻犬源大肠埃希菌耐药性以及整合子–基因盒检测研究[J].华南农业大学学报,2018,39(3):6-12. |
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| 作者姓名： | 魏斌 欧红萍 严光文 田一男 但佳明 涂蕊 肖启程 朱子琦 唐丽 杨亭玉 彭广能 钟志军 |
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| 作者单位： | 四川农业大学动物医学院/动物疫病与人类健康四川省重点实验室;成都农业科技职业学院;西昌学院动物科学学院 |
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| 基金项目： | 国家自然科学基金（31000548）；国家重点研发计划（2016YFD0501009）；成都大熊猫繁育研究基金（CPF2015-4）；四川农业大学本科生科研兴趣培养项目（ky2016076） |
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| 摘 要： | 【目的】了解腹泻犬源大肠埃希菌Escherichia coli的耐药性以及整合子携带情况。【方法】采用K-B纸片扩散法对30株分离自腹泻犬的大肠埃希菌进行19种抗菌药物的敏感性试验;PCR检测菌株中是否携带Ⅰ、Ⅱ、Ⅲ型整合酶基因,对携带有整合酶基因的阳性菌株进一步检测sul1、qac EΔ1基因以及可变区基因盒携带情况。【结果】30株分离株对19种抗菌药物表现出不同程度的耐药性,共产生18种耐药谱,对阿莫西林、氨苄西林、四环素和多西环素的耐药性较高,耐药率分别为90.00%、83.33%、66.67%和63.33%;对其余药物耐药性较低,耐药率低于14.00%;11株分离株含有Ⅰ型整合酶基因且均携带sul1和qacEΔ1基因,未检出Ⅱ型和Ⅲ型整合酶基因;Ⅰ型整合子阳性菌株中,有2株扩增出1 879 bp的耐药基因盒:dfrA12+orfF+aadA2。【结论】本次检测的腹泻犬源大肠埃希菌对抗菌药物呈不同水平的耐药性;基因盒介导的耐药性与菌株的耐药表型存在部分相关性,大肠埃希菌的耐药性与Ⅰ型整合子存在一定关系。
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| 关 键 词： | 犬 大肠埃希菌 耐药性 K-B纸片法 整合子–基因盒 |
| 收稿时间： | 2017/9/27 0:00:00 |
Antimicrobial resistance and integron-gene cassette detection of Escherichia coli isolates from diarrhea dogs |
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| WEI Bin,OU Hongping,YAN Guangwen,TIAN Yi''nan,DAN Jiaming,TU Rui,XIAO Qicheng,ZHU Ziqi,TANG Li,YANG Tingyu,PENG Guangneng and ZHONG Zhijun.Antimicrobial resistance and integron-gene cassette detection of Escherichia coli isolates from diarrhea dogs[J].Journal of South China Agricultural University,2018,39(3):6-12. |
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| Authors: | WEI Bin OU Hongping YAN Guangwen TIAN Yi'nan DAN Jiaming TU Rui XIAO Qicheng ZHU Ziqi TANG Li YANG Tingyu PENG Guangneng and ZHONG Zhijun |
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| Affiliation: | College of Veterinary Medicine, Sichuan Agricultural University/Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China,Chengdu Agricultural College, Chengdu 611130, China,College of Animal Science and Technology, Xichang College, Xi chang 615000, China,College of Veterinary Medicine, Sichuan Agricultural University/Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China,College of Veterinary Medicine, Sichuan Agricultural University/Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China,College of Veterinary Medicine, Sichuan Agricultural University/Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China,College of Veterinary Medicine, Sichuan Agricultural University/Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China,College of Veterinary Medicine, Sichuan Agricultural University/Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China,College of Veterinary Medicine, Sichuan Agricultural University/Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China,College of Veterinary Medicine, Sichuan Agricultural University/Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China,College of Veterinary Medicine, Sichuan Agricultural University/Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China and College of Veterinary Medicine, Sichuan Agricultural University/Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China |
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| Abstract: | |
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| Keywords: | dog Escherichia coli resistance Kirby-Bauer disc diffusion method integron-gene cassette |
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