| 下司犬线粒体DNA的遗传多样性研究 |
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| 引用本文： | 张嘉伟,冉雪琴,王嘉福.下司犬线粒体DNA的遗传多样性研究[J].贵州大学学报(农业与生物科学版),2009,28(2). |
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| 作者姓名： | 张嘉伟 冉雪琴 王嘉福 |
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| 作者单位： | 1. 贵州大学,动物科学学院,贵州,贵阳,550025
2. 贵州大学,动物科学学院,贵州,贵阳,550025;贵州大学,农业工程省重点实验室,贵州,贵阳,550025 |
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| 摘 要： | 为调查下司犬种群的遗传资源,采用PCR扩增和测序方法获得10只下司犬线粒体D-loop序列,发现其D-loop 5' 端高变区Ⅰ 582bp碱基序列的A+T含量明显高于G+C含量,表现出强烈的反G偏倚.并于下司犬高变区Ⅰ序列检测到11个多态位点,构成7种单倍型,单倍型多样性为0.867,说明下司犬线粒体D-loop的单倍型类型丰富,但其高变区Ⅰ的核苷酸多样性(Pi=0.00519)和平均核苷酸差异(k=3.002)却提示,下司犬的遗传多样性较低.聚类分析结果显示,中国家犬有3个母系来源,下司犬起源于Clade Ⅰ并与藏獒的亲缘关系最近,因此藏獒可用于对下司犬的选育和复壮.
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| 关 键 词： | 下司犬 线粒体DNA 遗传多样性 |
Genetic Diversity of Mitochondrial DNA Sequences in Xiasi Dog |
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| ZHANG Jia-wei,RAN Xue-qin,WANG Jia-fu.Genetic Diversity of Mitochondrial DNA Sequences in Xiasi Dog[J].Journal of Mountain Agriculture & Biology,2009,28(2). |
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| Authors: | ZHANG Jia-wei RAN Xue-qin WANG Jia-fu |
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| Affiliation: | 1.Faculty of Animal Science;Guizhou University;Guizhou Guiyang 550025;China;2.Guizhou Key Laboratory of Agricultural Bioengineering;China |
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| Abstract: | To investigate the genetic resource of Xiasi dog,the mitochondrial D-loop sequences of 10 individuals were obtained by Polymerase Chain Reaction(PCR).The hypervariable region I(582bp) at the 5' terminus of D-loop contained higher percentage of A+T than C+G due to anti-G bias,and seven haplotypes with abundant diversity(Hd=0.867) were defined by a total of 11 polymorphic sites from Xiasi dog.The nucleotide diversity and average number of nucleotide differences were 0.00519 and 3.002,respectively,indicating t... |
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| Keywords: | D-loop |
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