不同浓度外源过氧化氢对小菊扦插生根的影响

以小菊"东莞红"为试验材料,用0、50、100、200、500和1000μmol·L-1不同浓度外源H2O2对小菊插穗处理,观测插穗生根的发生和生长,测定叶片过氧化物酶(POD)和多酚氧化物酶(PPO)活性的变化。结果表明:外源过氧化氢处理对小菊插穗生根有明显的浓度效应,适宜浓度的外源过氧化氢能促进小菊插穗生根以及根的生长,过氧化氢浓度在50-500μmol·L-1的处理能促进插穗生根和根的生长,降低叶片POD活性,提高PPO活性,其中200μmol·L-1为最适宜浓度。

Effect of different hydrogen peroxide（H2O2） concentrations on rooting of little chrysanthemum cutting

To take chrysanthemum（Dendranthema morifolium-＇Dongguanhong＇.）as test material,different concentrations of H2O2（H2O2：0、50、100、200、500 and 1000μmol·L^-1）were used to understand the effects of hydrogen peroxide on rooting of plant cuttings,and the activity changes of Peroxidase（POD）and Polyphenol Oxidase（PPO）of cutting leaves were tested. The results showed that H2O2 on rooting of little chrysanthemum cuttings had concentration effect,with a maximal biological response at 200μmol·L^-1 H2O2,50μmol·L^-1 H2O2500μmol·L^-1. Treatments could enhance rooting and root growth synergistically and independently by stimulating the activities of PPO enzymes,and simultaneously by repressing POD activities.