| 10个棉花品种的DNA多态性分析 |
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| 引用本文： | 王建书,朱美霞,韩改英.10个棉花品种的DNA多态性分析[J].河南农业科学,2009(5). |
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| 作者姓名： | 王建书 朱美霞 韩改英 |
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| 作者单位： | 河北工程大学农学院,河北,邯郸,056021 |
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| 基金项目： | 河北省教育厅科学研究计划资助项目 |
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| 摘 要： | 采用RAPD技术,对10个棉花品种基因组进行DNA片断扩增,以研究品种间遗传多样性。结果表明:20个随机引物中,有8个引物能得到多态性条带。8个引物共扩增出96条带,其中33条多态性条带,多态率为34.4%。RAPD分析结果表明,用G06、G08、G11、G12、G17 5种引物可完全将这10个棉花品种区分开。此外,通过扩增BT基因发现,在非抗虫棉亚洲棉中也有BT基因的存在。
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| 关 键 词： | 棉花 随机扩增多态性DNA 遗传多样性 |
Analysis of Genetic Diversity among 10 Varieties of Cotton Based on RAPD Markers |
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| WANG Jian-shu,ZHU Mei-xia,HAN Gai-ying.Analysis of Genetic Diversity among 10 Varieties of Cotton Based on RAPD Markers[J].Journal of Henan Agricultural Sciences,2009(5). |
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| Authors: | WANG Jian-shu ZHU Mei-xia HAN Gai-ying |
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| Affiliation: | College of Agriculture;Hebei University of Engineering;Handan 056021;China |
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| Abstract: | The technique of random amplified polymorphic DNA(RAPD)was used to amplify genomic DNA of 10 cotton varieties.A total of 96 bands were amplified with 8 polymorphic primers and the polymorphic rate was 34.4%(33/96).The results showed that 5 kinds of random primers can differentiate the 10 varieties.In addition,there was also BT gene in G.arboreum of non-resis-tant-pest cottons that was amplified BT gene. |
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| Keywords: | Cotton Random amplified polymorphic DNA Genetic diversity |
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