| 荔波野生金线莲芽的诱导及其增殖 |
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| 引用本文： | 宋丽莎,邓伟,文治瑞,李玉红,吴丽娟,钱祥鑫.荔波野生金线莲芽的诱导及其增殖[J].贵州农业科学,2011(10):43-46. |
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| 作者姓名： | 宋丽莎 邓伟 文治瑞 李玉红 吴丽娟 钱祥鑫 |
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| 作者单位： | 黔南民族师范学院生命科学系;黔南职业技术学院; |
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| 基金项目： | 贵州省教育厅自然科学招标项目“喀斯特地区药用植物金线莲仿野生人工种植研究”[黔教科(2011)041]; 黔南州中药现代化建设项目“植物组织培养重点实验室”[黔南科技发字(2009)03] |
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| 摘 要： | 为探寻金线莲组织快繁的配套技术,以金线莲茎段、茎尖为外植体,研究NAA、6-BA浓度梯度及配比、不同部位外植体对不定芽诱导和增殖的影响.结果表明:MS+6-BA 1.0 mg/L+NAA 0.1 mg/L为适宜的诱导芽和增殖培养基,培养50 d后增值系数达6.5,MS+6-BA 1.0 mg/L+NAA 0.15 mg...
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| 关 键 词： | 金线莲 外植体 无菌系 增殖 |
Bud Induction and Propagation of Wild Anoectonchilus roxburghii from Libo County,Guizhou |
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| SONG Li-sha,DENG Wei,WEN Zhi-rui,LI Yu-hong,WU Li-juan,QIAN Xiang-xin.Bud Induction and Propagation of Wild Anoectonchilus roxburghii from Libo County,Guizhou[J].Guizhou Agricultural Sciences,2011(10):43-46. |
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| Authors: | SONG Li-sha DENG Wei WEN Zhi-rui LI Yu-hong WU Li-juan QIAN Xiang-xin |
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| Affiliation: | SONG Li-sha1,DENG Wei2,WEN Zhi-rui1,LI Yu-hong1,WU Li-juan1,QIAN Xiang-xin1(1.Department of Life Sciences,Qiannan Normal College for Nationalities,Duyun,Guizhou 558000,2.Qianna Vocational and Technical College,China) |
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| Abstract: | The stem sections and shoot tips of A.roxburghii were cultured on the medium with different NAA and 6-BA concentration and proportion to study the effects on bud induction and propagation of different explants for building a set of technology of tissue propagation in A.roxburghii.The results showed that the optimum medium for bud induction and propagation of stem sections and shoot tips of A.roxburghii was MS+1.0 mg/L 6-BA+0.1 mg/L NAA and the propagation coefficient could reach 6.5 after 50 d.The optimum m... |
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| Keywords: | Anoectonchilus roxburghii explant sterile line propagation |
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