油菜素内酯与CaCl2复配对复合逆境下黄瓜幼苗的诱抗作用

为了明确油菜素内酯(Brassinolide,BR)与氯化钙(Calcium Chloride,CaCl2)复配对盐胁迫、灰霉病菌侵染复合逆境下黄瓜(Cucumis sativusL.)幼苗的诱抗作用,采用根际注射结合叶面喷洒的方法,测定了复合逆境下黄瓜幼苗的盐害和病害指数、保护酶活性和抗病相关物质含量等生理指标。结果表明:BR与CaCl2复配处理使黄瓜幼苗的盐害和病情指数最高分别比对照降低了91.04%、17.89%;提高了黄瓜叶片中脯氨酸和木质素含量,最高分别比对照增加了24.99%、22.66%;降低了电解质渗出率和丙二醛(MDA)含量,最高分别比对照降低了43.81%、50.52%;增强了超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、苯丙氨酸解氨酶(PAL)和多酚氧化酶(PPO)活性。综合作用效果,BR与CaCl2复配优于BR或CaCl2单一处理。

EFFECTS OF BRASSINOLIDE AND CACL2 MIXTURE ON THE INDUCED RESISTANCE OF CUCUMBER SEEDLINFS UNDER COMPOUND STRESS

Root injection and foliar spray were adopted to study induced resistance of brassinolide(BR) and CaCl2 mixture on cucumber(Cucumis sativus L.) seedlings under salt and Botrytis cinerea Pers.compound stress,we determined salt injure and disease index,protective enzyme activities and disease resistance-related substance contents in cucumber seedling.The results showed that BR and CaCl2 mixture decreased salinity injure and disease index of cucumber seedlings by 91.04%,17.89% compared with control,increased Pro and lignin content by 24.99%,22.66%,decreased permeation rate of electrolyte and malondialdehyde(MDA) content by 43.81%,50.52%,improved superoxide dismutase(SOD),catalase(CAT),peroxidase(POD),phenylalanine ammonia lyase(PAL) and polyphenol oxidase(PPO) activities.Induced resistance effect of BR and CaCl2 mixture was better than single BR or CaCl2.