| 美人指葡萄再生体系的建立 |
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| 引用本文： | 余智莹,张平,任俊鹏,章镇,袁红燕,陶建敏.美人指葡萄再生体系的建立[J].江西农业学报,2011,23(5):44-49. |
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| 作者姓名： | 余智莹 张平 任俊鹏 章镇 袁红燕 陶建敏 |
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| 作者单位： | 南京农业大学园艺学院,江苏南京,210095 |
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| 基金项目： | 江苏省自主创新基金项目 |
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| 摘 要： | 以美人指葡萄为试材,研究了不同外植体类型、激素浓度配比以及暗培养时间等对器官再生的影响。结果表明:叶片、叶柄、茎段外植体均可再生出不定芽,其中叶柄的再生率最高;TDZ对美人指葡萄叶柄再生效果比6-BA好,IBA诱导美人指葡萄叶柄不定芽再生率高于NAA和2,4-D;暗培养3周时,美人指葡萄叶柄的再生率最高。叶片再生的最适培养基为:MS+1.0 mg/L TDZ+0.1 mg/L IBA,再生率为60.71%,增殖系数为5.65;叶柄再生的最适培养基为:MS+1.0mg/LTDZ+0.1 mg/L IBA,再生率为93.10%,增殖系数为5.91;茎段再生的最适培养基为:MS+1.0 mg/L TDZ+0.2 mg/L IBA,再生率为47.37%,增殖系数为5.00。将再生不定芽置于3/4 MS+0.35 mg/L IBA培养基上诱导生根,完整植株培养35~50 d后炼苗移栽,成活率可达90%。
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| 关 键 词： | 美人指葡萄 器官再生 不定芽 再生植株 |
Regeneration of Grape ' Manicure Finger' (Vitis vinifera) from Its Isolated Explants |
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| YU Zhi-ying,ZHANG Ping,REN Jun-peng,ZHANG Zhen,YUAN Hong-yan,TAO Jian-min.Regeneration of Grape ' Manicure Finger' (Vitis vinifera) from Its Isolated Explants[J].Acta Agriculturae Jiangxi,2011,23(5):44-49. |
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| Authors: | YU Zhi-ying ZHANG Ping REN Jun-peng ZHANG Zhen YUAN Hong-yan TAO Jian-min |
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| Affiliation: | (College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China) |
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| Abstract: | The effects of different explant types,different hormones and their concentration combinations as well as dark culture time on the regeneration of grape Manicure Finger (Vitis vinifera) were studied.The results showed that adventitious buds could regenerate from the isolated leaves,petioles and stem segments;among them the regenerated rate of adventitious buds from the isolated petioles was maximum.TDZ enhanced the regeneration of adventitious buds from petioles better than 6-BA.IBA was better than NAA and... |
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| Keywords: | ‘Manicure Finger’ grape Organogenesis Adventitious bud Regenerated plantlet |
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