转扁桃AcCBF1基因对烟草光合及叶绿素荧光特性的影响

【目的】扁桃AcCBF1基因对光合作用的影响,为扁桃新品种选育提供科学依据。【方法】采用农杆菌介导法获得转AcCBF1基因烟草,在经过连续室外37℃高温天气一周后,以野生烟草为对照,测定和分析试验烟草的光合参数和叶绿素荧光参数日变化特性。【结果】利用农杆菌介导法成功获得3株转基因烟草,经PCR检测证明AcCBF1基因已稳定地整合到烟草基因组中。转基因烟草和野生烟草的Pn、Gs均呈现“双峰”曲线,且峰值出现时间一致。12:00达到第一个峰值,16:00出现光合“午休”,18:00达到第二个峰值。二者的Pn日均值分别为6.496 、4.553 μmol/(m²·s);Gs日均值分别为157.357 、133.371 μmol/(m²·s)。转基因烟草和野生烟草的Fo均呈现出先上升后下降的趋势,Fm呈现先下降后上升的趋势。Fv/Fm、Fv/Fo、ΦPSII与Fm日变化趋势一致,均呈现先下降后上升的趋势,二者的Fv/Fm日均值分别为0.821、0.736;Fv/Fo日均值分别为5.044、3.391、ΦPSII日均值分别为0.763、0.600。【结论】与野生烟草相比,转AcCBF1基因烟草同化CO₂能力较高,光合能力较强,光合反应中心较稳定,对高温的适应能力较强。扁桃AcCBF1基因能够有效改善烟草的光合作用,维护光合反应中心的稳定;选育扁桃AcCBF1高表达品种应对夏季的干旱高温。

Study on Photosynthetic and Chlorophyll Fluorescence Characteristics of Transgenic Tobacco with AcCBF1 Gene

【Objective】 To explore the effects of AcCBF1 gene on photosynthesis, and provide a scientific basis for breeding new varieties of almonds.【Method】 In this experiment, the transgenic tobacco with AcCBF1 gene was obtained by Agrobacterium-mediated method. After a week of continuous outdoor high temperature weather at 37 ℃, the diural variation characteristics of photosynthetic parameters and chlorophyll fluorescence parameters were measured and analyzed with the wild tobacco as control.【Result】 In this experiment, three transgenic tobaccos were successfully obtained by Agrobacterium-mediated method, and PCR analysis showed that the AcCBF1 gene had been stably integrated into the tobacco genome. Both Pn and Gs of transgenic tobacco and wild tobacco showed a "double peak" curve and peaks appeared at the same time. The first peak appeared at 12:00, The phenomenon of "mid-day depression" occurred at 16:00, and the second peak appeared at 18:00. The daily average values of Pn were 6.496 and 4.553 μ mol/(m²·s), respectively ; the daily average values of Gs were 157.357 and 133.371 μ mol/(m²·s) , respectively. The Fo of transgenic tobacco and wild tobacco showed a trend of increasing first and then decreasing, and Fm showed a trend of decreasing first and then increasing. The daily variation trends of Fv/Fm, Fv/Fo, and ΦPSII were the same, and they all showed a trend of decreasing first and then increasing. The daily average values of Fv/Fm values were 0.821 and 0.736, respectively. The daily average values of Fv/Fo were 5.044 and 3.391, respectively. The daily average values of ΦPSII were 0.763 and 0.600, respectively.【Conclusion】By analyzing and comparing the changes of photosynthetic parameters and fluorescence chlorophyll parameters, we found that transgenic AcCBF1 tobacco has higher assimilation capacity of CO₂, stronger photosynthetic ability, more stable photosynthetic reaction center and stronger adaptability to high temperature comparing with wild tobacco. To cope with drought and high temperature in summer, we could select and breed new almond varieties with AcCBF1 high-expression.