浙江省新昌县茶叶炭疽病病原鉴定及其药剂敏感性测定

为了确定浙江新昌茶叶炭疽病的病原菌,采用组织分离法对其进行病原菌分离和病害特征描述,通过对病原菌形态结构特征的观察、致病性的试验和rDNA ITS序列的测定比较,对病原菌进行了鉴定,并针对该病原菌采用生长速率法进行了药剂敏感性测定。病原鉴定结果表明,引起浙江省新昌县的茶叶炭疽病的病原菌为胶孢炭疽菌(Colletotrichun gloeosporioides);15种药剂敏感性测定结果表明,250 g·L^-1吡唑醚菌酯乳油、65%代森锌可湿性粉剂、45%咪鲜胺微乳剂、250 g·L^-1丙环唑乳油和70%甲基硫菌灵可湿性粉剂对茶叶炭疽病菌丝生长具有较强的抑制效果(EC₅₀<1 mg·L^-1);325 g·L^-1苯甲·嘧菌酯悬浮剂、10%苯醚甲环唑水分散颗粒剂、430 g·L^-1戊唑醇悬浮剂、38%唑醚·啶酰菌水分散颗粒剂和10%己唑醇悬浮剂的抑制效果次之(1 mg·L^-150<10 mg·L^-1);12.5%烯唑醇可湿性粉剂、25 g·L^-1咯菌腈悬浮种衣剂和42.8%氟菌·肟菌酯悬浮剂的抑制效果相对较差(EC₅₀>10 mg·L^-1);50%多菌灵可湿性粉剂和50%啶酰菌胺水分散颗粒剂对茶叶炭疽病菌丝生长基本无效。

Pathogen identification and susceptibility to fungicides on tea anthracnose in Xinchang of Zhejiang Province

Tissue isolation method was used to isolate the pathogens from the symptomatic leaves of tea which were collected from Xinchang of Zhejiang Province. These isolates were identified into Colletotrichun gloeosporioides based on its morphology, pathogenicity and rDNA ITS sequence. Fungicide sensitivity for this pathogen was tested in this study. The results showed that C. gloeosporioides is more sensitive to 250 g·L^-1 pyraclostrobin, 65% zineb, 45% prochloraz, 250 g·L^-1 propiconazole and 70% thiophanate-methyl (EC₅₀<1 mg·L^-1), followed by 325 g·L^-1 difenoconazole·azoxystrobin, 10% difenoconazole, 430 g·L^-1 tebuconazole, 38% pyraclostrobin·boscalid and 10% hexaconazole (1 mg·L^-150<10 mg·L^-1). The sensitivity of C. gloeosporioides to 12.5% diniconazole, 25 g·L^-1 fludioxonil and 42.8% fluopyram·trifloxystrobin were lower than the others (EC₅₀>10 mg·L^-1). Moreover, the mycelium of C. gloeosporioides can growth well with 50% carbendazim and 50% boscalid.