不同种质番茄材料抗番茄黄化曲叶病毒病特性研究

以不同种质番茄为实验材料,利用苗期农杆菌接种法接种番茄黄化曲叶病毒(tomato yellow leaf curl virus, TYLCV),采用实时定量PCR和生理生化方法评价对TYLCV的抗性,统计分析不同抗性番茄材料不同发病时期的表型特征、带毒率、抗氧化酶活性、总酚与类黄酮含量及抗性基因表达量的变化情况,并分析各抗性指标之间的关系。结果表明,番茄材料带毒率为100%;853没有任何感病症状,802与803感病最为严重;抗氧化酶活性、总酚与类黄酮含量及抗性基因表达量在各时期均较高的有867、857、853,817与842次之,803最低。SOD活性、CAT活性、APX活性、总酚含量、类黄酮含量、Ty-1基因、Ty-2和Ty-3基因的表达量相互之间均呈极显著正相关(总酚与Ty-1之间为显著正相关,P<0.01)。番茄植株感染TYLCV后,Ty-1的表达相比于其他抗病基因具有延迟性。综合分析筛选出867、857、853三种抗病性较强的材料,植株体内的抗性基因、抗氧化酶、总酚与类黄酮之间呈正相关,共同提高了番茄植株对TYLCV的抗性。

Study on resistance of different tomato germplasm materials to yellow leaf curl virus disease

Different tomato materials were inoculated with tomato yellow leaf curl virus (TYLCV) by Agrobacterium tumefaciens infiltration. Real-time quantitative PCR and physiological and biochemical methods were used to evaluate the resistance to TYLCV. The phenotypic characteristics, virus carrying rate, antioxidant enzyme activity, secondary metabolite content and resistance gene expression of tomato materials with different resistance were analyzed. The results showed that the virus infection rates of tomato materials were 100%; the tested variety 853 had no symptoms, while 802 and 803 were the most susceptible; the varieties 867, 857 and 853 had higher antioxidant enzyme activity, contents of total phenols and flavonoids, and higher resistance gene expression, followed by 817 and 842, the lowest was 803. SOD activity, CAT activity, APX activity, total phenol content, flavonoid content, Ty-1 gene, Ty-2 and Ty-3 gene expression levels were significantly positively correlated with each other (total phenol and Ty-1 showed significantly positive correlation, P<0.01). Compared with other disease resistance genes, the expression of Ty-1 was delayed after tomato plants were infected with TYLCV. The resistance genes, antioxidant enzymes, total phenols and flavonoids in plants were significantly positively correlated, which jointly improved the resistance of tomato plants to TYLCV.