转甜菜碱醛脱氢酶基因水稻种子萌发和苗期耐盐性的研究

用0.0、3.0、5.0g/L NaCl对2个转甜菜碱醛脱氢酶(BADH)基因水稻51-22、52-7与受体亲本中花8进行萌发期盐胁迫试验.结果表明,盐胁迫下转基因材料受抑程度小于受体亲本,尤以52-7表现明显.用0.0、3.0、5.0g/L NaCl对51-22、52-7和中花8在幼苗期进行14 d盐胁迫试验.结果表明,低盐胁迫时幼苗受盐害较小,高盐胁迫时转基因材料耐盐性优于受体亲本;在盐胁迫7 d后,转基因材料体内脯氨酸含量明显高于受体亲本,同一品种随盐度增大,脯氨酸含量升高.

A Study On Seed Germination and Seedling Salt-tolerance of the Transformed Betaine Aldehyde Dehydrogenase Gene Rice

Experiments under salt-stress are carried out between the two transformed Betaine Aldehyde Dehydrogenase(BADH) Gene Rice 51-22 & 52-7 and the receptor parent Zhonghua 8 during germination period using consistan gradient of NaCl at 0.0,3.0,5.0 g/L.The results showed that: transgenic strains undergo less inhibition than their receptor parent under salt stress,especially remarkable in 52-7.When gradient of NaCl is set at 0.0,3.0,5.0,7.0 g/L,experiment in salty condition again is done between 51-22,52-7 and zhonghua 8 for 14 days during seedling stage.As it's shown,the seedlings suffer less when the gradient is low and the transgenic strains has a supperiority to their parent when it's high.7 days after salt stressing,Prolin content in transgenic strains rise greatly higher than those of the receptor parent.What's more,the Proline will in crease with salinity rise for the same variety.