瓣化型胡萝卜胞质雄性不育相关片段的分离及序列分析

以128对引物组合对瓣化型胡萝卜胞质雄性不育系和保持系进行cDNA-AFLP分析，筛选与不育性相关的特异片段，对其中两个表达丰度较高的cDNA差异片段进行克隆测序。序列分析表明BY1949与拟南芥质子依赖性的类肽转运蛋白在核苷酸水平有87%的同源性，蛋白质水平有58%的同源性。BY2562与瓣化型胞质雄性不育（CMS）胡萝卜线粒体ATP8、ATP9、NAD6和cob-sp基因在核苷酸水平有99%的同源性，期望值为0.0；在蛋白质水平与CMS向日葵线粒体ORFB基因，COXIII 5'端未知蛋白YMF19以及CMS胡萝卜ATP-8，orfB-F1有97%的同源性；另外与胡萝卜orfB-CMS、orfB-F2、烟草orfB、油菜orf224、拟南芥线粒体orfB、萝卜orf158以及芥菜线粒体膜蛋白、甘蓝型油菜orf474也有90%以上的同源性。

Cloning and Seqence Analysis of Fragments Related to Cytoplasmc Male Sterility in Petaloid type carrot

cDNA-AFLP analysis was developed on male sterile line and maintainer line in carrot with 128 pairs of AFLP primers combination, in order to screen out specific fragments related to sterility. Two differential fragments were cloned and sequenced. sequence analysis at nucleotide acid level indicates that BY1949 has 87% identity with proton-dependent peptide transporter-like protein from Arabidopsis thaliana, and that has 58% identity at the protein level. And at nucleotide acid level BY2562 has 99% identity with atp8, atp9, nad6 and cob-Sp allele in CMS carota, Expect = 0.0. At the protein level that has 97% identity with ORFB gene and YMF19 of CMS common sunflower mitochondrion, as well as ATP-8,orfB-F1 in CMS carrot. In addition the identity is over 90% with orfB protein of Nicotiana tabacum, orf224 of Brassica napus, orf158 of radish, orfB of Arabidopsis thaliana and mitochondrial membrane associated protein of Brassica juncea.