| 外源信号物质对肉苁蓉种子萌发与吸器形成POD、CAT活性及ROS含量的影响 |
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| 引用本文： | 陈虞超,李 苗,巩 檑,宋玉霞.外源信号物质对肉苁蓉种子萌发与吸器形成POD、CAT活性及ROS含量的影响[J].中国农学通报,2014,30(36):128-132. |
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| 作者姓名： | 陈虞超 李 苗 巩 檑 宋玉霞 |
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| 作者单位： | 宁夏农业生物技术重点实验室,宁夏农业生物技术重点实验室,宁夏农业生物技术重点实验室,宁夏农业生物技术重点实验室 |
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| 基金项目： | 基金项目：国家自然科学基金项目“肉苁蓉专性寄生外源信号物质作用研究(30960023)，“肉苁蓉属植物与寄主间水平基因转移研究”(31160066)；宁夏科技支撑计划项目“药用濒危植物肉苁蓉人工控制寄生技术研究”(2013ZYS148)；宁夏自然科学基金项目“肉苁蓉离体寄生体系的建立(NZ12262)。 |
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| 摘 要： | 利用外源信号物质氟草敏(norflurazon)和2,6-二甲氧基对苯醌(2,6-DMBQ)分别诱导肉苁蓉种子萌发与吸器形成，研究它们在此过程中对过氧化物酶(POD)、过氧化氢酶(CAT)活性及活性氧(ROS)含量的影响。结果表明：在诱导肉苁蓉种子萌发时，经norflurazon处理0~168 h后，种子中POD和CAT活性呈现显著升高的变化趋势，种子中ROS含量显著升高。在诱导肉苁蓉种子萌发体吸器形成时，经2,6-DMBQ处理0~72 h后，肉苁蓉种子萌发体中POD和CAT活性呈现显著升高的变化趋势，萌发体中ROS含量显著升高。表明在诱导肉苁蓉种子萌发与吸器形成过程中，外源信号物质norflurazon和2,6-DMBQ能影响POD、CAT活性及ROS含量的变化。
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| 关 键 词： | 外源信号物质 肉苁蓉 种子萌发 吸器形成 POD CAT ROS |
| 收稿时间： | 5/5/2014 12:00:00 AM |
| 修稿时间： | 2014/10/23 0:00:00 |
Effects of Exogenous Signal Substances on POD, CAT Activity and ROS Content in Cistanche deserticola Y. C. Ma Seeds During Germination and Haustorium Formation |
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| Chen Yuchao,Li Miao,Gong Lei and Song Yuxia.Effects of Exogenous Signal Substances on POD, CAT Activity and ROS Content in Cistanche deserticola Y. C. Ma Seeds During Germination and Haustorium Formation[J].Chinese Agricultural Science Bulletin,2014,30(36):128-132. |
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| Authors: | Chen Yuchao Li Miao Gong Lei and Song Yuxia |
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| Affiliation: | Key Laboratory of Agricultural Biotechnology of Ningxia,Key Laboratory of Agricultural Biotechnology of Ningxia,Key Laboratory of Agricultural Biotechnology of Ningxia,Key Laboratory of Agricultural Biotechnology of Ningxia |
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| Abstract: | The seed germination and haustorium formation of Cistanche deserticola were induced by norflurazon and 2,6-dimethoxy-p-benzoquinone (2,6-DMBQ) respectively. The effects of them on peroxidase(POD), catalase(CAT) activities and reactive oxygen species(ROS) content during seed germination and haustorium formation were studied. The results showed that POD, CAT activities and ROS content significantly increased during 0-168 h treated with norflurazon. POD, CAT activities and ROS content significantly increased during 0-72 h treated with 2,6-DMBQ. The results preliminarily confirmed that the exogenous signal substances, norflurazon and 2,6-DMBQ, effected POD, CAT activities and ROS content of C. deserticola during seed germination and haustorium formation. |
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| Keywords: | exogenous signal substances Cistanche deserticola seed germination haustorium formation POD CAT ROS |
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