内生真菌GG22诱导子对地黄毛状根生长和次生代谢的影响

为探索不同浓度内生真菌GG22诱导子与地黄毛状根共培养不同时间后,对毛状根的生长及次生代谢产物合成的影响,本试验通过制备地黄内生真菌GG22诱导子溶液,加入已培养23 d的毛状根液体培养基中,使含诱导子的多糖浓度分别为0(对照)、10、30、50和100 mg·L^-1,共培养0、3、5、8、11 d后,分别测定地黄毛状根的鲜重、干重、总环烯醚萜苷及毛蕊花糖苷的含量。结果表明,加入诱导子溶液的试验组毛状根的鲜重、干重、总环烯醚萜苷及毛蕊花糖苷的含量比对照组高。且通过变异系数权重法分析综合得分,得出最适宜诱导条件为诱导子浓度50 mg·L^-1,共培养11 d。由此推测地黄内生真菌GG22诱导子在一定诱导浓度和培养天数下能够促进地黄毛状根的生长,并提高其次生代谢产物的含量。本试验结果为进一步研究地黄内生真菌GG22诱导子促进地黄次生代谢产物积累的分子机制提供了基础。

Effects of Endophytic Fungal GG22 Elicitor on Growth and Secondary Metabolism of Hairy Roots of Rehmannia glutinosa

In order to explore the effects of endophytic fungus GG22 inducer of Rehmannia glutinosa on the growth of hairy roots and the synthesis of secondary metabolites such as total iridoid glycosides and acteoside of R. glutinosa, the induction effect of co-culture of hairy root of R. glutinosa with different concentrations of elicitor for different time was preliminarily studied. In this experiment, the inducer solution of endophytic fungus GG22 of R. glutinosa was prepared and added into the liquid culture medium of hairy roots which had been cultured for 23 days, the total sugar concentration in the co-culture medium was 0, 10, 30, 50 and 100 mg·L^-1 respectively. After co-culture for 0, 3, 5, 8 and 11 days, the fresh weight, dry weight, total iridoid glycoside and verbascoside content of hairy roots of R. glutinosa were determined respectively. The results showed that the fresh weight, dry weight, total iridoid glycoside and verbascoside content of hairy roots in the experimental group were higher than those in the blank control group. And with the comprehensive score analyzed by the coefficient of variation weight method, the most suitable induction condition is that co-cuture for 11 d with 50 mg·L^-1 elicitor. Therefore, it can be speculated that the elicitor of endophytic fungus GG22 of R. glutinosa can promote the growth of hairy roots of R. glutinosa and increase the content of its secondary metabolites under certain induction concentration and culture days.This study would provide a basis for further study on the molecular mechanism of the inducer of endophytic fungus GG22 promoting the accumulation of secondary metabolites of R. glutinosa.