| 岩溶地貌部位对土壤微生物丰度与酶活性的影响 |
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| 引用本文： | 李强,靳振江,李忠义,罗堃,唐志琴,黄静云,陆文体.岩溶地貌部位对土壤微生物丰度与酶活性的影响[J].水土保持通报,2014(3):50-54. |
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| 作者姓名： | 李强 靳振江 李忠义 罗堃 唐志琴 黄静云 陆文体 |
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| 作者单位： | 国土资源部/广西岩溶动力学重点实验室中国地质科学院岩溶地质研究所, 广西桂林 541004;国土资源部/广西岩溶动力学重点实验室中国地质科学院岩溶地质研究所, 广西桂林 541004;广西环境污染控制理论与技术重点实验室桂林理工大学环境科学与工程学院, 广西桂林 541004;广西农业科学院农业资源与环境研究所, 广西南宁 530007;广西环境污染控制理论与技术重点实验室桂林理工大学环境科学与工程学院, 广西桂林 541004;广西环境污染控制理论与技术重点实验室桂林理工大学环境科学与工程学院, 广西桂林 541004;广西环境污染控制理论与技术重点实验室桂林理工大学环境科学与工程学院, 广西桂林 541004;广西环境污染控制理论与技术重点实验室桂林理工大学环境科学与工程学院, 广西桂林 541004 |
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| 基金项目： | 中国地质调查局项目"百名青年地质英才培养计划"; 国家自然科学基金项目"岩溶干旱过程中钙对忍冬光合生理的调控机制"(41003038); 广西自然科学基金项目(2011GXNSFD018002), (2010GXNSFB013004), (2011GXNSFA018006) |
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| 摘 要： | 以中国地质科学院岩溶地质研究所桂林丫吉岩溶试验场为研究区,单氨加氧酶的编码基因amoA的部分序列作为氨氧化细菌(AOB)指示基因,利用荧光定量PCR技术,结合平板菌落计数法和土壤酶活性测定,探讨了岩溶地貌部位及土壤成因对微生物群落丰度和酶活性的影响。结果发现,受岩溶地貌部位和土壤成因的影响,土壤酶活性、土壤微生物总数在垭口、坡地、洼地呈增加的趋势,而氨氧化细菌丰度则呈现降低的趋势。结果表明,土壤氨氧化细菌丰度与真菌数呈极其显著的正相关,而与蔗糖酶活性呈极其显著负相关。
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| 关 键 词： | 岩溶地貌 酶活性 微生物群落丰度 荧光定量PCR 氨氧化细菌 |
| 收稿时间： | 2013/3/25 0:00:00 |
| 修稿时间： | 2013/6/21 0:00:00 |
Effect of Karst Physiognomy on Soil Microbial Abundance and Enzyme Activities |
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| LI Qiang,JIN Zhen-jiang,LI Zhong-yi,LUO Kun,TANG Zhi-qin,HUANG Jing-yun and LU Wen-ti.Effect of Karst Physiognomy on Soil Microbial Abundance and Enzyme Activities[J].Bulletin of Soil and Water Conservation,2014(3):50-54. |
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| Authors: | LI Qiang JIN Zhen-jiang LI Zhong-yi LUO Kun TANG Zhi-qin HUANG Jing-yun and LU Wen-ti |
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| Affiliation: | Key Laboratory of Karst Dynamics of the MLR and Guangxi Province, Institute of Karst Geology, CAGS, Guilin, Guangxi 541004, China;Key Laboratory of Karst Dynamics of the MLR and Guangxi Province, Institute of Karst Geology, CAGS, Guilin, Guangxi 541004, China;College of Environmental Science and Engineering, Guilin University of Technology, Guilin, Guangxi 541004, China;Institute of Agricultural Resources and Environment, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, China;College of Environmental Science and Engineering, Guilin University of Technology, Guilin, Guangxi 541004, China;College of Environmental Science and Engineering, Guilin University of Technology, Guilin, Guangxi 541004, China;College of Environmental Science and Engineering, Guilin University of Technology, Guilin, Guangxi 541004, China;College of Environmental Science and Engineering, Guilin University of Technology, Guilin, Guangxi 541004, China |
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| Abstract: | The Yaji experimental site of Institute of Karst Geology, Chinese Academy of Geology Science, was selected as the study area to discuss the effects of karst physiognomy on soil microbial abundance and enzyme activity. The gene abundances of amoA, as the index of ammonia-oxidizing bacteria(AOB) communities, were determined by real-time quantitative PCR(qPCR), while microbial community population and soil enzymes were analyzed using dilute plate incubation counting and colorimetric methods, respectively. Results show that the enzyme activities and total microbial numbers of soils in saddle back, piedmont slope and karst depression increased while the soil AOB decreased. Moreover, the abundances of AOB communities had a significantly positive correlation with fungi, while it had a significantly negative correlation with sucrase activity. |
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| Keywords: | karst physiognomy enzyme activity microbial community abundance real-time PCR ammonia-oxidizing bacteria |
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