| 贝氏高原鳅不同组织原代培养的研究 |
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| 引用本文： | 黄静,王志坚,霍静.贝氏高原鳅不同组织原代培养的研究[J].西南师范大学学报(自然科学版),2011,36(5). |
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| 作者姓名： | 黄静 王志坚 霍静 |
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| 作者单位： | 淡水鱼类资源与生殖发育教育部重点实验室水产科学重庆市市级重点实验室西南大学生命科学学院; |
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| 摘 要： | 采用胰酶半消化法,对贝氏高原鳅的吻端、肾脏和性腺组织的原代培养条件进行探究,使用MEM,RPMI1640,DMEM和DMEM/F12培养基进行培养;血清质量分数设置为10%,30%,50%和100%;分别置于37℃,26℃和室温(11～15℃)条件下培养,观察细胞在不同条件下的生长情况.初步确定了各组织原代培养的最佳条件是温度26℃、血清质量分数50%的条件下,吻端和肾脏组织在MEM培养基、精巢在DMEM培养基中生长情况最优.Giemsa染色显示各组织细胞结构完整,荧光染料Alexa fluor 488Phalloidin显示各组织细胞的微丝网络发达.
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| 关 键 词： | 贝氏高原鳅 组织 原代培养 条件优化 微丝 |
Primary Culture of Different Tissues from Triplophysa bleekeri (Sauvage et Dabry) |
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| HUANG Jing,WANG Zhi-jian,HUO Jing Key Laboratory of Freshwater Fish Reproduction , Development,Ministry of Education,Key Laboratory of Aquatic Science of Chongqing,School of Life Science,Southwest University,Chongqing ,China.Primary Culture of Different Tissues from Triplophysa bleekeri (Sauvage et Dabry)[J].Journal of Southwest China Normal University(Natural Science),2011,36(5). |
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| Authors: | HUANG Jing WANG Zhi-jian HUO Jing Key Laboratory of Freshwater Fish Reproduction Development Ministry of Education Key Laboratory of Aquatic Science of Chongqing School of Life Science Southwest University Chongqing China |
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| Affiliation: | HUANG Jing,WANG Zhi-jian,HUO Jing Key Laboratory of Freshwater Fish Reproduction and Development,Ministry of Education,Key Laboratory of Aquatic Science of Chongqing,School of Life Science,Southwest University,Chongqing 400715,China |
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| Abstract: | |
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| Keywords: | Triplophysa bleekeri tissue primary culture optimized condition microfilament |
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