表儿茶素没食子酸酯对人鼻咽癌C666-1细胞凋亡的影响

该文观察ECG在人鼻咽癌细胞株C666-1增殖与凋亡中的作用,并初步探讨其机制.人鼻咽癌C666-1细胞给予不同浓度ECG(0~100μmol/L)处理48 h后,采用CCK-8法检测细胞增殖水平,使用TUNEL法和流式细胞仪检测细胞凋亡率,利用Western blotting法检测Bax、Bcl-2、Caspase 3等凋亡相关蛋白的表达变化.结果表明,25μmol/L以上浓度的ECG可抑制C666-1细胞增殖,诱导细胞凋亡;并且ECG可浓度依赖性增加Bax/Bcl-2蛋白比值和剪切的Caspase 3蛋白水平.以上结果表明ECG可抑制人鼻咽癌细胞株C666-1增殖,并诱导细胞凋亡.ECG的作用与其增加Bax/Bcl-2相对蛋白比值进而激活caspase-3有关.

Effect of Epicatechin Gallate on the Apoptosis of Human Nasopharyngeal Carcinoma Cell Line C666-1

The effect of ECG on the proliferation and apoptosis of nasopharyngeal carcinoma cell line C666-1 were studied. C666-1 cells were treated with different concentrations (0~100 mol/L) of ECG for 48 h, CCK-8 assay was used to evaluate the proliferation. TUNEL assay and Flow Cytometric Analysis were used to investigate the apoptosis of C666-1 cells. Western blotting was performed to detect the expression level of Bax, Bcl-2 and Caspase 3. ECG above 25 mol/L could inhibit the proliferation, induce the apoptosis of C666-1 cells, and upregulate the levels of Bax/Bcl-2 and cleaved caspase 3. ECG could inhibit proliferation and induce apoptosis of nasopharyngeal carcinoma cell line C666-1 by regulating Bax-Bcl-2-Caspase 3 signaling pathway.