| 牦牛肝片吸虫四种抗原交叉免疫印迹分析 |
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| 引用本文： | 乔代蓉,赵坚.牦牛肝片吸虫四种抗原交叉免疫印迹分析[J].四川大学学报(自然科学版),1997,34(1):111-114. |
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| 作者姓名： | 乔代蓉 赵坚 |
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| 摘 要： | 用牦牛肝片吸虫四种抗原分别制备四种抗血清，其ＥＳＩＳＡ效价 １：８００，１：１６００，１：３２００和１：８００．ＳＤＳ－ＰＡＧＥ电泳区带组分分别为２５，２４，１８和９条带；免疫印迹结果，各种抗清与相庆抗原作用的主要抗原成份分别为５，１０，７和３；四种抗原中存在的共同抗原为１７．２ｋＤ；特征抗原ＨＡ为２９．９ｋＤ，ＳＡ为２７．２ｋＤ的２４．３ｋＤ；正常兔血清与各个抗原间均未出现免疫反应。
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| 关 键 词： | 牦牛 免疫印迹 肝片吸虫病 抗原 寄生虫病 |
ANALYSIS OF FOUR PARTS PROTEINS AND ANTIGENS OF YAK F.HEPATICA BY MEANS OF SDS PAGE AND CROSS IMMUNOBLOTTING |
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| Qiao,Dairong,Zhao,Jian,Cheng Shuqiu Liu Shigui.ANALYSIS OF FOUR PARTS PROTEINS AND ANTIGENS OF YAK F.HEPATICA BY MEANS OF SDS PAGE AND CROSS IMMUNOBLOTTING[J].Journal of Sichuan University (Natural Science Edition),1997,34(1):111-114. |
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| Authors: | Qiao Dairong Zhao Jian Cheng Shuqiu Liu Shigui |
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| Affiliation: | National Lab of Grassland Biotechnology |
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| Abstract: | |
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| Keywords: | F heptica yak SDS PAGE immunoblotting |
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