海口地区人乳头瘤病毒感染的分子流行病学研究

利用PCR扩增和Southen杂交技术，对海口地区人乳头瘤病毒（HPV）型别进行了调查，结果显示，在所抽取的1045份样本中，有436份检出HPV-DNA，总阳性率为41．72％（436／1045），其中有337份只检出1种型别感染（单侵染），65份检出2种型别感染（二重侵染），34份检出3种或3种以上型别感染（三重或三重以上侵染），分别占感染样本的77．29％，14．91％和7．80％；在单侵染中，高危型所占比例为74．78％（252／337）,不同型别的检出率从高到低分别是HPV16（22．25％），HPV58（17．89％），HPV43（16．74％），HPV52（16．06）和HPV6（8．94％）．其中HPV16，HPV58和HPV43为高危型，其检出率较高，具有一定的区域性．

Molecular Epidemiology of Human Papillomavirus (HPV) Infection in Haikou Area

In the report, southern blot and PCR were performed to investigate the main genotypes of Human Pap-illomavirus （HPV） in Haikou area. The results indicated that among 1 045 samples, 436 cases were positive （41.72%）, and among which the single infection cases, double infection cases, and multiple infection cases were 337 （77.29%）, 65 （ 14.91% ） and 34 （7.80%）, respectively; the proportion of high-risk HPV geno-type was 74.78% （252/337） in single infection; the most frequently detected HPV genotypes were HPV16, HPV58, HPV43, HPV52 and HPV6 with ratio of 22.25% , 17.89%, 16.74%, 16.06% and 8.94% , respec-tively, among which HPV16, HPV58, and HPV43 were high-risk genotypes, all of which suggested that Haikou region has its own HPV distribution characteristic.