| 土壤中PVA降解菌的分离与筛选 |
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| 引用本文： | 强雪妮,郭雅妮,杨贞.土壤中PVA降解菌的分离与筛选[J].西安工程科技学院学报,2014(3):325-328. |
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| 作者姓名： | 强雪妮 郭雅妮 杨贞 |
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| 作者单位： | 西安工程大学环境与化学工程学院; |
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| 基金项目： | 陕西省科技厅科学技术研究发展计划项目(2010K02-10);陕西省教育厅专项科研计划项目(09JK448) |
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| 摘 要： | 以含PVA的土壤作为菌种来源，PVA为唯一碳源，在相同培养条件下，采用I2-KI及硼酸染色法进行初筛，再通过测定PV A 降解效率的方法进行复筛。初筛得到PV A 降解菌6株，复筛得到4株单一的PV A降解菌A ，C ，E ，F ，降解效率在48％～61％之间。通过对4株PV A降解单菌进行生理生化实验鉴定出A为假单胞菌属，C为球菌属，E为微球菌属，F为链球菌属。
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| 关 键 词： | 聚乙烯醇 混合菌系 筛选 土壤 |
Isolation and screening of different strains for degradation of PVA in soil |
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| QIANG Xue-ni,GUO Ya-ni,YANG Zhen.Isolation and screening of different strains for degradation of PVA in soil[J].Journal of Xi an University of Engineering Science and Technology,2014(3):325-328. |
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| Authors: | QIANG Xue-ni GUO Ya-ni YANG Zhen |
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| Affiliation: | (School of EnvironmenTal and Chemical Engineering, Xi＇an Polyrechnic University, Xi＇an 710048, China) |
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| Abstract: | Taking PVA contained soil as the strains source ,the I2-KI and boric acid staining method are used for primary screen when PVA is sole carbon source under the same culture conditions .The strains are secondary screened through the determination of PVA degradation efficiency .Six strains are ob-tained through primary screening and four strains are obtained through secondary screening .The PVA degradation efficiency of four strains （A ,C ,E ,F） is between 48%-61% .The A ,C ,E and F strins are Pseudomonas ,Cocci ,Micrococcus and Streptococcus ,respectively . |
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| Keywords: | PVA mixed strains screening soil |
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