| 离舌橐吾根部的抗肿瘤活性成分研究 |
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| 引用本文： | 徐 栋,刘 丹,张晨欢,张润芳,王钦莲,邹 坤,王 慧.离舌橐吾根部的抗肿瘤活性成分研究[J].华中师范大学学报(自然科学版),2021,55(1):72-75. |
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| 作者姓名： | 徐 栋 刘 丹 张晨欢 张润芳 王钦莲 邹 坤 王 慧 |
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| 作者单位： | 1.三峡大学生物与制药学院天然产物研究与利用湖北省重点实验室(三峡大学)，湖北 宜昌 443002；2.三峡大学生物与制药学院中国轻工业功能酵母重点实验室，湖北 宜昌 443002 |
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| 摘 要： | 综合运用硅胶柱、TLC和制备HPLC等多种色谱技术，从离舌橐吾Ligularia veitchiana(Hemsl.) Greenm根部分离得到了7个单体化合物，并根据理化性质和波谱数据鉴定其结构分别为：bakkenolide A(1)，phthalic acid isodibutyl ester(2)，dibutyl phthalate(3)，eremophila-7 (11),9-dien-8-one(4),6β，8β-hydroxyeremophil-7(11)-en-12,8α-olide(5),6β-hydroxyeremophil-7(11)-en-12,8β-olide(6)和6β-Hydroxyeremophil-7(11)-en-12,8α-olide(7).其中，化合物1、2、4和7为首次从该植物中分离得到.化合物1为首个从离舌橐吾中分离得到的具有螺环骨架的倍半萜类化合物.化合物6与化合物7为一对非对映异构体.采用MTT法对所有化合物针对人胃癌细胞株(HGC-27)的体外抗肿瘤细胞抑制活性进行评价.结果表明化合物1对人胃癌细胞(HGC-27)具有显著的细胞毒抑制活性，其IC50为31.10 μg·mL－1.
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| 关 键 词： | 离舌橐吾 化学成分 抗肿瘤活性 |
| 收稿时间： | 2021-01-13 |
Antitumor constituents from roots of Ligularia veitchiana |
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| XU Dong,LIU Dan,ZHANG Chenhuan,ZHANG Runfang,WANG Qinlian,ZOU Kun,WANG Hui.Antitumor constituents from roots of Ligularia veitchiana[J].Journal of Central China Normal University(Natural Sciences),2021,55(1):72-75. |
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| Authors: | XU Dong LIU Dan ZHANG Chenhuan ZHANG Runfang WANG Qinlian ZOU Kun WANG Hui |
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| Affiliation: | 1.Hubei Key Laboratory of Natural Products Research and Development， College of Biological and Pharmaceutical Sciences， China Three Gorges University， Yichang， Hubei 443002， China； 2.Key Laboratory of Functional Yeast， China National Light Industry， College of Biological and Pharmaceutical Sciences， China Three Gorges University， Yichang， Hubei 443002， China |
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| Abstract: | Various chromatographic techniques， including silica gel column chromatography， TLC and HPLC， were used to investigate the chemical constituents of roots of L. veitchiana.Three compounds were isolated and then identified as bakkenolide A (1)， phthalic acid isodibutyl ester (2)， dibutylphthalate (3)， eremophila-7(11),9-dien-8-one (4)， 6β，8β-hydroxyeremophil-7(11)-en-12,8α-olide (5)， 6β-hydroxyeremophil-7(11)-en-12,8β-olide (6)， and 6β-hydroxyeremophil-7(11)-en-12,8α-olide (7) by means of spectroscopic and chemical data. Among them， compounds 1，2，4 and 7 were isolated from this plant for the first time.Compound 1 was the first sesquiterpenoid with a spiro-ring skeleton isolated from L. veitchiana. Compounds 6 and 7 area pair of diastereoisomer. Cytotoxicities of all the compounds against cancer cell lines of HGC-27 were evaluated in vitro by MTT assay， and compound 1 showed a significant growth inhibition of tumor cells HGC-27 with IC50 of 31.10 μg·mL－1. |
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| Keywords: | Ligularia veitchiana chemical constituents antitumor activity |
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