锁阳多糖对运动训练大鼠血清酶活性与尿蛋白含量的影响

探讨锁阳多糖(Cynomrium songaricum Rupr polysaccharide,CSRP)对运动训练大鼠骨骼肌、心、肝、肾等组织细胞及线粒体的保护作用,为CSRP在运动医学中的应用提供实验依据.采用分光光度法测定安静对照组、运动训练组和运动+CSRP组大鼠血清琥珀酸脱氢酶(succinatedehydrogenase,SDH)、肌酸激酶(creatine kinase,CK)、乳酸脱氢酶酶(lactate dehydrogenase,LDH)、丙氨酸转氨酶(alanine transaminase,ALT)、天冬氨酸转氨酶(aspartate aminotransferase,AST)活性和尿液总蛋白(total protein,TP)、尿液白蛋白(albumin,Alb)和尿液β2-微球蛋白(β2-microglobulin,β2-MC)含量等相关生物化学指标;跑台法测定运动训练组和运动+CSRP组大鼠运动至力竭的时间.结果表明:运动+CSRP组血清SDH、CK、LDH活性显著低于运动对照组(P<0.05),ALT、AST活性显著低于运动对照组(P<0.05),TP、Alb和β2-MC含量显著低于运动对照组(P<0.05);运动+锁阳多糖组大鼠运动至力竭的时间与运动对照组比较明显延长(P<0.05).表明CSRP可以降低血清SDH、CK、LDH、ALT、AST活性和TP、Alb和β2-MC含量,保护运动训练大鼠骨骼肌、心、肝、肾等不同组织细胞和线粒体的结构,维持其正常功能,可延长大鼠跑台运动至力竭时间,具有抗疲劳作用.

Effect of Cynomrium songaricum Rupr polysaccharide on activity of serum and content of urinary protein in exercise training rats

Effect of Cynomrium songaricum Rupr polysaccharide(CSRP)on protection role to skeletal muscle,heart,liver,kidney and other tissue cells and mitochondria in exercise training rats,for CSRP application in sports medicine and provide experimental basis.Using spectrophotometry to measure serum succinate dehydrogenase succinate dehydrogenase(SDH)、 creatine kinase(CK)、 lactate dehydrogenase enzyme(LDH)、 alanine aminotransferase(ALT),aspartate aminotransferase(AST) activity and urine total protein(TP)、 urinary albumin(Alb) and urine fluid β2-microglobulin(β2-MC) content and other related biochemical indicators in rats of quiet control group,exercise training group and the exercise + CSRP group;treadmill exercise to measure exercise time to exhaustion in rats of training group and determined campaign + CSRP.The results showed: exercise + CSRP serum SDH、CK、LDH activity was significantly lower than the exercise control group(P<0.05);exercise + CSRP serum ALT、 AST activity was significantly lower than the exercise control group(P<0.05);exercise + CSRP group of TP、 Alb,and β2-MC was significantly lower than the exercise control group(P<0.05);exercise + Cynomorium polysaccharide rats exercise and exercise time to exhaustion of the control group was significantly longer(P<0.05).The results show that CSRP can reduce the serum SDH、 CK、 LDH、 ALT、 AST activity and TP、 Alb and β2-MC content,to protect rats muscle、heart、liver、kidney and other tissue cells and mitochondria of different structures,to maintain its normal Function,may be extended to exhaustive treadmill exercise time with antifatigue effect.