EB1蛋白在调节微管动态、纺锤体定位和染色体稳定性中的作用

EB1(the end-binding protein 1)蛋白家族是一群广泛存在且高度保守的微管相关蛋白,存在于从酵母到人类的广泛的生物体中.它与微管正极和中心体结合,参与了绝大部分基于微管的生理过程,包括:维持细胞极性,调节染色体稳定性,有丝分裂纺锤体的定位,将微管锚定到成核位点.自从1995年,Su等人在人细胞中发现了EB1基因,各种生物体中EB1的同源物质被相继报道.十年来,人们通过对不同生物体的研究,试图揭开EB1在细胞中的分布以及它的生理功能.然而到目前为止,对于EB1的了解还非常有限.本文结合国外的研究成果,对EB1蛋白在调节微管动态、纺锤体定位和染色体的稳定性方面以及它与APC(the adenomatous polyposis coli)之间的相互作用作以综述.

EB1 modulate microtubule dynamics,spindle localization and chromosome maintenance

EB1 proteins are ubiquitous microtubule-associated proteins.The EB1 family represents a highly conserved group of proteins,presents in from yeast to humans,binds microtubule plus-ends and centrosome.EB1 proteins are implicated in most MT-based processes,including maintenance of cell polarity,regulation of chromosome stability,positioning of the mitotic spindle,and anchoring of MTs to their nucleation sites.All kinds of homologies of EB1 have been reported since Su et al.found EB1 gene in human in 1995.During ten years,people try to understand the distribution and function of EB1 in different organisms.However, up to now,our knowledge of EB1 is still limited.Combined with the results of research abroad,in this review,we focus on the functions of EB1 proteins in which regulates microtubule dynamics,cell polarity,chromosome stability and on the physical interaction with the adenomatous polyposis coli(APC) tumor suppressor protein.