木霉菌拌种对小麦生长和根际真菌群落的影响

为了了解木霉菌拌种对冬小麦生长和根际土壤真菌群落的影响,选取小麦种植后180 d的根际土壤,通过高通量测序技术研究分析了4株木霉菌拌种对小麦根际土壤中的真菌群落组成的影响,通过qPCR检测了根际土壤中真菌的绝对含量。结果表明：木霉菌拌种处理后降低了小麦根际土壤中真菌群落丰度,同时发现小麦产量与小麦根际土壤真菌群落均匀度(香农指数)和丰富度(OTUs数)呈反比,而与群落的优势度(辛普森指数)呈正比；通过田间病害调查和测产,结果显示,与未拌种处理相比木霉菌拌种降低了小麦纹枯病和茎基腐病引起的白穗率,其中哈茨木霉LTR-2和QT21990防效可达60% 以上,增产7.42%和6.94%(P<0.05)。

Effects of Seed Dressing with Trichoderma spp. on Wheat Grown and Rhizosphere Fungal Community

A field experiment was conducted to investigate effects of seed dressing with Trichoderma spp. on wheat growth and rhizosphere fungal community. The wheat rhizosphere soil was collected after sowing 180 days, and the effects of four different strains of Trichoderma on fungal community in wheat rhizosphere soil were studied by high-throughput sequencing technology, the absolute content of fungi was detected by qPCR. The results showed that the abundance of fungal community in wheat rhizosphere soil was decreased by Trichoderma spp. treatment. The wheat yield was inversely proportional to the uniformity (Shannon index) and richness (OTUs number) of fungal community in rhizosphere soil, but positively proportional to the dominance (Simpson index). The white head rate, caused by wheat sharp eyespot and crown rot, was decreased compared with non-dressing treatment. The control effects of Trichoderma harzianum LTR-2 and QT21990 were more than 60%, and the yield increased by 7.42% and 6.94% (P<0.05), respectively.