| 中国栗属特有种保守RAPD片段分析 |
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| 引用本文： | 沈永宝,施季森.中国栗属特有种保守RAPD片段分析[J].南京林业大学学报(自然科学版),2004,28(4):51-53. |
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| 作者姓名： | 沈永宝 施季森 |
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| 作者单位： | 南京林业大学国家林业局南方林木种子检验中心,江苏,南京,210037 |
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| 基金项目： | 国家林业局重点资助项目 ( 2 0 0 0 -3 6) |
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| 摘 要： | 利用RAPDDNA分子标记技术研究了栗属特有种(板栗、茅栗和锥栗)特异保守片段。结果表明，从520多个随机引物中筛选出有扩增产物，且多态性丰富的10个引物，其中引物B08(GTCCACACGG)分别在所有供试的板栗、茅栗和锥栗中均扩增出各自特异的DNA片段，其片段大小分别为2050，900和1500bp。这些DNA片段在种水平上的高度保守性，可以用来区分这3个种。此次研究结果同时也说明RAPD标记是鉴别近缘种的理想工具。
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| 关 键 词： | RAPD标记 板栗 锥栗 茅栗 鉴定 保守DNA片段 |
| 文章编号： | 1000-2006(2004)04-0051-03 |
| 修稿时间： | 2003年9月29日 |
Analysis on Conservative DNA Segment for Different Species of Castanea in China by RAPD Marker |
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| SHEN Yong-bao,SHI Ji-sen.Analysis on Conservative DNA Segment for Different Species of Castanea in China by RAPD Marker[J].Journal of Nanjing Forestry University(Natural Sciences ),2004,28(4):51-53. |
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| Authors: | SHEN Yong-bao SHI Ji-sen |
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| Abstract: | Identification method of plant species mainly done according to their morphologic characters can not be applied to close species or their parts of tissue.However,development of DNA molecular markers during the past more than 10 years made it possible.This paper studies the application of RAPD molecular marker in distinguishing 3 different species following Castanea mollissima,C.henyi and C.seguinii.The results shown that different special conservative segment of each species was enlarged with primer B07(GTCCACACGG).Respectively three species-specific DNA segments weighted of 2 050 bp,900 bp and 1 500 bp.This method can be used in species identification widely. |
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| Keywords: | RAPD marker Castanea mollissima C henyi C seguinii Identification Conservative segment of DNA |
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