|
|||||
|
|
| 外源hDAF基因对转基因猪影响的初步研究 | |
| 引用本文： | 陈付学,魏庆信,曹军平,郑新民,郭晖,陈实,张英汉.外源hDAF基因对转基因猪影响的初步研究[J].上海大学学报(自然科学版),2001,7(3):249-252. |
| 作者姓名： | 陈付学 魏庆信 曹军平 郑新民 郭晖 陈实 张英汉 |
| 作者单位： | 1. 上海大学生命科学学院, 2. 湖北农科院畜牧所, 3. 同济医科大学附属器官移植研究所, 4. 西北农林科技大学动物科学与动物学学院, |
| 基金项目： | 国家863计划(101-05-05-01)资助项目 |
| 摘 要： | 应用显微注射方法将外源hDNA基因导入湖北白猪的受精卵，获得了相应的转基因猪，4．2，8．0kb两种导入外源基因片断移植后死胎率分别为20．00％，39．71％，差异不显著，8．0kb基因产仔畸形率为11．76％，而4．3kb基因无畸形，结果表明，原核基因对转基因猪有明显的致畸作用，G0代转基因猪60日龄重，6月龄重对照组差异均不显著，表明外源基因的整合位点较理想。 |
| 关 键 词： | 转基因猪 显微注射 移植 外源hDAF基因 纹畸作用 整合位点 |
| 文章编号： | 1007-2861(2001)03-0249-04 |
| 修稿时间： | 2001年1月9日 |
Effect of Foreign hDAF Genes on Transgenic Pigs |
|
| CHEN Fu xue ,WEI Qing xin ,CAO Jun ping ,ZHENG Xin min ,GUO Hui ,CHEN Shi ,ZHANG Ying han.Effect of Foreign hDAF Genes on Transgenic Pigs[J].Journal of Shanghai University(Natural Science),2001,7(3):249-252. | |
| Authors: | CHEN Fu xue WEI Qing xin CAO Jun ping ZHENG Xin min GUO Hui CHEN Shi ZHANG Ying han |
| Affiliation: | CHEN Fu xue 1,WEI Qing xin 2,CAO Jun ping 2,ZHENG Xin min 2,GUO Hui 3,CHEN Shi 3,ZHANG Ying han 4 |
| Abstract: | The foreign gene of hDAF was microinjected into fertilized eggs of Hubei Big Pigs, and transgenic pigs were obtained. The stillbirth rate was 20.00% and 39.71% respectively with two genes (4.3 kb and 8.0 kb). The difference was insignificant. The malformation rate of 8.0 kb gene transgenic pigs was 11.76%, but no malformation was found in 4.3 kb gene transgenic pigs. There was no difference in weight at 60 days and 6 months of transgenic pigs and controls, which indicated that integrated sites were proper. |
| Keywords: | Transgenic pigs hDAF Microinjection |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
