免疫PCR方法检测乳腺癌患者血清抗p53蛋白抗体

目的为乳腺癌诊断提供血清学新方法.方法免疫PCR方法检测血清抗p53蛋白抗体,酶免疫组化方法检测组织p53蛋白表达.结果乳腺癌患者血清抗p53蛋白抗体阳性率为39.5%,而非癌患者和正常人血清抗p53蛋白抗体均为阴性,乳腺癌患者血清中抗p53蛋白抗体显著高于非癌患者和正常人(P＜0.01).p53蛋白阳性表达的乳腺癌患者抗p53蛋白抗体阳性率为64.2%,明显高于p53蛋白阴性表达组,血清p53抗体测定与p53蛋白表达密切相关(P＜0.01).结论检测血清抗p53蛋白抗体是检测组织p53蛋白理想的替代工具抗p53蛋白抗体可以作为乳腺癌血清学诊断新标志,用于乳腺癌的普查和早期诊断.

Detection of Seral p53 Antibody in Patients with Breast Carcinoma with Immuno-PCR

ObjectiveTo present a new seriological method for diagnosing breast carcinoma.MethodsSeral anti-p53 antibody was detected with immuno-PCR. The expression of p53 in tissue was detected with enzyme immunohistochemistry technique.ResultsThe positive rate of anti-p53 antibody in serum of breast carcinoma was (39.5%) and anti-p53 antibody was not found in non-carcinoma and normal controls. The positive rate of anti-p53 antibody was higher than that of non-carcinoma and normal controls( P <(0.01)). The positive rate of anti-p53 antibody in positive p53 expression was (64.2%) and was higher than that in negative p52 expression. Seral anti-p53 antibody related to p53 expression in tissue obviously( P <(0.01)).ConclusionDetecting seral anti-p53 antibody was alternative tool for detecting p53 expression in tissue. Seral anti-p53 antibody is a new marker for diagnosing breast carcinoma and can be used in early diagnosing breast carcinoma.