何首乌18S rRNA基因序列分析

目的 分析不同产地野生何首乌Fallopia multiflora(Thunb.) Harald及种植品种和伪品芭蕉的核基因组18S rRNA序列,为探讨野生和种植品种物种间的亲缘关系和鉴别提供分子依据 方法 采用PCR直接测序技术测定野生和种植的何首乌及芭蕉的18S rRNA基因核苷酸序列并作序列变异分析 结果 野生和种植的何首乌及芭蕉的18SrRNA序列长度均为 1809bp,根据排序比较,只有来自广西德保、广西药用植物园和云南野生植物18SrRNA基因序列在680、1712和1724有相同的碱基替代，其余的序列完全相同； 与何首乌基因序列相比较，伪品芭蕉有71个位点发生碱基置换，在284和1537位置上分别插入了C和A碱基，而在135和500位置分别缺失了T和A 结论 通过较保守的基18SrRNA基因序列同源性分析,基本可以认为野生品种和种植品种基原一致，可用于何首乌的真伪鉴定。

18SrRNA Nucleotide Sequencing of Fallopia Multiflora(Thunb.) Harald.

Object: To study the relation of cultivars and wildness Fallopia multiflora (Thunb.) Harald from different geography by sequencing their nuclear ribosomal RNA subunit (18SrRNA), and to provide molecular evidences for identify the drugs from Musa Basjoo Sieb.&Zucc.. Methods: By direct PCR sequencing to detect the homology of 18SrRNA sequence of specimens. Results: The 18SrRNA genes of specimens have the identical length of 1809. Multiple sequences alignment of 18SrRNA gene of Fallopia Multiflora (Thunb.) Harald shown that they have the same sequence except the specimens from Debao of Guangxi province, Guangxi officinal arboretum and Yunnan province that the nucleotide substitutions were observed at position 680,1712 and 1724.Compare with Fallopia Multiflora (Thunb.) Harald sequences, Musa Basjoo Sieb.&Zucc was found 71nucleotide substitutions, deletion of T&A at position 135 and 500 and insertion of C&A at 284 and 1537，respectively. Conclusion: Based on the homology of 18SrRNA gene, the cultivars and wildness Fallopia multiflora (Thunb.) Harald have the identical botanical origins, which is a reliable mean for its identification.