新疆黑白轮枝菌与大丽轮枝菌核糖体基因ITS区的克隆和序列分析

采用真菌核糖体基因内转录间隔区域(ITS)通用引物ITS1和ITS4扩增黑白轮枝菌与大丽轮枝菌核糖体基因的ITS,并对PCR产物进行了克隆和序列比较。实验结果表明,黑白轮枝菌和大丽轮枝菌的ITS区都由541个碱基组成;供试的2个黑白轮枝菌菌株的ITS碱基序列同源性为100%,供试的2个大丽轮枝菌的ITS碱基序列同源性为99.82%,有1个碱基的差别。黑白轮枝菌和大丽轮枝菌核糖体ITS碱基序列同源性为99.40%,供试的黑白轮枝菌与供试的大丽轮枝菌至少有6个碱基的差别,且差异所在区域较集中。

Clone and Analysis of Sequence of Ribosome Gene on the Internal Transcribed Spacer in V.albo-atrum and V.dahliae

The V.albo-atrun and V.dahliae's ribosome gene ITS were amplified by the method of using universal primers ITS1 and ITS4 of fungus internal transcribed spacer(ITS).The products of PCR were cloned and the sequence were compared.The results showed that the ITS of V.albo-atrum and V.dahliae is made up of 541 bp respectively.The sequence homology of two V.albo-atrum strains' ribosome gene ITS bp is 100%.The sequence homology of two V.dahliae's ribosome gene ITS bp is 99.81%.The sequence homology between the V.albo-atrum ribosome gene ITS bp and the V.dahliae ribosome gene ITS bp is 99.40%.There are six bps difference between the V.albo-atrum ribosome gene ITS bp and the V.dahliae ribosome gene ITS bp and the area of the difference is concentrative.