吸入白细胞介素-5对支气管哮喘患者外周血嗜酸性粒细胞数及其活化状态的影响

随机选择8例哮喘患者雾化吸入人重组IL-5,分别吸入前、吸入后2 h、24 h和48 h进行外周血有核细胞计数和分类,以放射免疫法测定血清中嗜酸性阳离子蛋白(ECP)及总IgE水平.结果,吸入IL-5后嗜酸性粒细胞(EOS)占细胞总数的百分比、EOS绝对细胞数以及ECP水平均随时间而明显升高,至24 h达最高值,48 h后仍维持在较高水平;IL-5在整个实验过程中对血清总IgE水平无明显影响.表明,吸入IL-5不仅可以促使循环中EOS数明显增多,且还能招致其活化从而参与哮喘的发病过程.

Effect of Inhaled Interleukin-5 on Activity and Number of Eosinophils in Circulation from Asthmatics

For examining the effects of recombinant human(rh)IL-5 inhalation on changes of activity and number of circulating eosinophils in allergic asthmatics, as well as concentrations of serum IgE,a randomized double-blind, placebo-controlled study design was employed, in which each subject acted as his or her own control. Eight nonsmoking patients with allergic asthma were administered rhIL-5 by nebulization, total blood nuc lear cell counts and differentials, as well as concentrations of ECP and IgE in serum were determined before, and at 2 h,24 h,48 h after inhalation, respectivel y.Eosinophil numbers and ECP levels within the control group did not appear to change from baseline at any time throughout the study, eosinophil numbers from baseline(3.6±1.1×105/mL)to 6.3±1.2×105/mL (P<0.01) at 24 h,and to 5.7±0.9×105/mL(P<0.01)at 48h after IL-5 inhalation. Accompa nying this significant blood eosinophilia was a significant elevation of serum E CP levels. Compared with baseline value(6.3±1.1 pg/mL), inhalation of IL-5 lead to ECP levels increase with time, reaching 17.6±2.8 pg/mL at 24 h(P<0.01); and this elevated ECP levels lasted at least 48 h(18.1±2.9 pg/ mL,P<0.01).IL-5 inhalation had no significant effect of levels of serum to tal IgE.our findings provided direct evidence that IL-5 not only induced a significant blood eosinophilia, but also resulted in the activation of circulating eosinophils.