| 顺铂对卵巢癌细胞几种生长和凋亡相关基因表达的影响 |
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| 引用本文： | 张政希,艾晓杰,李伟.顺铂对卵巢癌细胞几种生长和凋亡相关基因表达的影响[J].复旦学报(自然科学版),2011(3):373-377. |
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| 作者姓名： | 张政希 艾晓杰 李伟 |
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| 作者单位： | 上海交通大学系统生物医学研究院上海系统生物医学研究中心教育部系统生物医学重点实验室;上海交通大学农业与生物学院; |
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| 基金项目： | 上海市纳米专项基金(05dz22321); 上海市自然科学基金(11ZR1416800) |
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| 摘 要： | 顺铂是治疗卵巢癌的一线药物，它与癌细胞内的DNA结合是产生细胞毒性的主要原因.在用基因表达谱芯片筛选顺铂处理IGROV-1细胞产生的差异表达基因基础上，选取其中与细胞生长、凋亡有关的部分重要基因，用实时定量PCR方法进行了差异表达验证.结果表明，顺铂可引起CEACAM1、KLF6、JUN、TP53INP1、MAP2K4...
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| 关 键 词： | 顺铂 差异表达基因 实时定量PCR 细胞凋亡 |
Effects of Cisplatin on the Expression of Several Growth and Apoptosis Related Genes in Ovarian Cancer Cells |
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| ZHANG Zheng-xi,AI Xiao-jie,LI Wei.Effects of Cisplatin on the Expression of Several Growth and Apoptosis Related Genes in Ovarian Cancer Cells[J].Journal of Fudan University(Natural Science),2011(3):373-377. |
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| Authors: | ZHANG Zheng-xi AI Xiao-jie LI Wei |
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| Affiliation: | ZHANG Zheng-xi1,AI Xiao-jie2,LI Wei1(1.Key Laboratory of Systems Biomedicne,Ministry of Education,Shanghai Center for Systems Biomedicine,Institute for Systems Biomedicine,Shanghai Jiao Tong Univercity,Shanghai 200240,China,2.School ofAgriculture and Biology,Shanghai Jiao Tong University,China) |
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| Abstract: | Cisplatin is one of the primary medicines used in ovarian cancer treatment,it produces cytotoxicity through binding with DNA.Based on the data obtained from gene expression array analysis,some important genes involved in cell growth,apoptosis were selected to validate the differential expression using real-time RT-PCR.The results showed that the expression of six genes was upregulated by cisplatin treatment,these proteins encoded by six genes included CEACAM1,KLF6,JUN,TP53INP1,MAP2K4 and UBE2D3,the expressi... |
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| Keywords: | Cisplatin differentially expressed gene real-time PCR cell apoptosis |
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