应用红细胞光敏模型评价抗光敏中药添加剂

 研究了紫外线或光敏药物单独存在的条件下以及不同照射时间和不同药物浓度条件对模型的影响。建立了长波紫外线诱导光敏药物致使红细胞溶血模型，通过检测红细胞损伤后上清液中的血红蛋白含量，计算溶血率，从而反映细胞损伤程度。结果表明，紫外线低剂量或氯丙嗪单一条件存在时，并不能引起红细胞溶血；当氯丙嗪含量为0.33mg/mL，紫外线照射30min可致红细胞50%溶血，照射60min可使细胞100%溶血。应用此模型对10种中药及植物成分进行检测，筛选出银杏叶提取液、茶多酚和葡萄籽提取液3种具有抗光敏功能的活性成分。其中，银杏叶中主要成分黄酮类物质通过广泛的紫外吸收能力从源头阻止紫外线对细胞膜的损伤，茶多酚、葡萄籽中的多酚类物质通过清除自由基等光产物对细胞膜起到保护作用。

Application of Photosensitive Model of Red Blood Cell to Evaluate the Anti-photosensitive Additives in Traditional Chinese Medicine

With the increase of UV-sensitive groups, more and more attention is paid on the problems of UV-sensitive skins. However, the methods to evaluate the photosensitive damage are more often than not for animal testing. In this paper, a relatively less time consuming and less expensive convenient method is developed to detect the damage of UV-A by red blood cell hemolysis, first to obtain the hemoglobin of the injured red blood cell, and then to calculate the hemolytic rate. The experiments on the effects of ultraviolet light or photo sensitizer alone, the different exposure times and the different drug concentrations show that the red blood cell hemolysis does not occur under ultraviolet light radiation alone without chlorpromazine nor only with chlorpromazine without ultraviolet light radiation. With the chlorpromazine content of 0.33mg/mL in red blood cell, 50% hemolysis can be resulted after being radiated by ultraviolet for about 30min, and 100% hemolysis after 60min of radiation. 10 kinds of Chinese medicine were found with the active ingredients of anti-photosensitive from ginkgo biloba leaf, tea polyphenols, grape seed extraction, as the most potential anti-photosensitive materials. Among them, the main components of ginkgo biloba flavonoids were shown be able to prevent the damage of UV on the cell membrane from the source through the broad UV absorption capacity, tea polyphenols and grape seed polyphenols could protect the cell membrane by scavenging free radicals and other optical products.