线粒体腺苷酸转运体在宫颈癌组织中的表达变化及其与细胞凋亡的关系

 为探讨线粒体腺苷酸转运体（ANT）在宫颈癌组织中的表达变化及其与细胞凋亡的关系，收集维吾尔族妇女浸润性宫颈鳞癌患者的组织36 例，正常或者慢性宫颈炎宫颈组织32 例作为对照。分别运用荧光定量PCR（Q-PCR）、蛋白免疫印迹（westernblot，WB）技术，检测线粒体腺苷酸转运体ANT1、ANT2、ANT3，以及Bax、Bcl-2、p53 在不同宫颈组织中的mRNA 和蛋白表达水平及其差异。运用TUNEL 法测定不同宫颈组织中的细胞凋亡程度。Q-PCR 和WB 结果显示，与正常对照组相比，p53、Bax 在宫颈癌患者宫颈组织中表达降低，而Bcl-2 和ANT3 表达升高，差异有统计学意义（P<0.05）。TUNEL 结果显示，宫颈癌组的凋亡指数（AI）明显高于正常对照宫颈炎组，两组之间差异有统计学意义（P<0.05）。线粒体腺苷酸转运体ANT 在宫颈癌组织中的异常表达导致线粒体功能紊乱，从而干扰细胞凋亡过程，这可能是宫颈癌发生与发展的一个重要机制。

Association of Cell Apoptosis with the Change of Expression of ANT in Tissue of Cervical Cancer

To investigate the association of the cell apoptosis with the expression change of adenine nucleotide translocator (ANT) in the tissue of Uighur women with cervical cancer, we collected cervical tissue samples from Uygur women with cervical lesions (invasive cervical cancer (ICC): 36 cases and cervicitis: 32 cases). The mRNA and protein expression levels of ANT1, ANT2, ANT3, Bax, Bcl-2, and P53 in the tissues from the two groups were examined by real-time fluorescence quantitative PCR (Q-PCR) and western blot (WB). Apoptotic activity of cervical epithelium cells from the two groups was studied by TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) method. The results of Q-PCR and WB show that compared with the cervicitis group, the expressions of p53 and Bax in the ICC group decreased while the expressions of Bcl-2 and ANT3 increased, and the difference was statistically significant (P<0.05). The TUNEL results showed that the apoptosis index (AI) of the ICC group was obviously higher than that of the normal control cervicitis groups, the difference between the two groups was statistically significant (P<0.05). The abnormal expression of ANT in cervical cancer leads to mitochondrial dysfunction, thus interfering the apoptosis process, which may be an important mechanism of the occurrence and development of cervical cancer.