| CONSTANS-LIKE 7基因对拟南芥开花的调控分析 |
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| 引用本文： | 王宏归,黄 晨,陈 鹏,姜 雅,张 娅,唐冬英,赵小英,刘选明.CONSTANS-LIKE 7基因对拟南芥开花的调控分析[J].湖南大学学报(自然科学版),2015,42(12):88-94. |
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| 作者姓名： | 王宏归 黄 晨 陈 鹏 姜 雅 张 娅 唐冬英 赵小英 刘选明 |
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| 作者单位： | (1.扬州大学 环境科学与工程学院，江苏 扬州 225127；2.湖南大学 生物学院，植物功能基因组学与发育调控湖南省重点实验室，湖南 长沙 410082） |
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| 摘 要： | 为研究CONSTANS-LIKE 7(COL7)在调控植物开花方面的功能,以定量PCR,GUS染色等方法,研究光及生物钟对COL7表达的影响,以及COL7对拟南芥开花的影响.实验结果显示:光及生物钟参与调控COL7的表达;过表达COL7在长日照条件下抑制CONSTANS(CO)以及FLOWERING LOCUS T(FT)的表达,进而抑制拟南芥开花;col7突变体不论是在长日照下还是短日照下都没有明显的开花表型,说明COL7在调控拟南芥开化方面可能与其家族基因中的其它成员存在功能冗余.
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| 关 键 词： | 基因表达 拟南芥 CONSTANS-LIKE 7 开花 |
The Analysis of CONSTANS-LIKE 7 Regulateing Arabidopsis Flowering Time |
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| WANG Hong-gui,HUANG Chen,CHEN Peng,JIANG Y,ZHANG Y,TANG Dong-ying,ZHAO Xiao-ying,LIU Xuan-ming.The Analysis of CONSTANS-LIKE 7 Regulateing Arabidopsis Flowering Time[J].Journal of Hunan University(Naturnal Science),2015,42(12):88-94. |
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| Authors: | WANG Hong-gui HUANG Chen CHEN Peng JIANG Y ZHANG Y TANG Dong-ying ZHAO Xiao-ying LIU Xuan-ming |
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| Abstract: | To discover the function of CONSTANS-LIKE 7 (COL7) in regulating plant flowering, we used qPCR, GUS staining, et al, to study the light and circadian regulating the expression of COL7, and COL7 regulating Arabidopsis flowering time. The results showed that COL7 was regulated by light and circadian, and the overexpressing COL7 transgenic lines inhibited Arabidopsis flowering time under long-day (LD) condition through down-regulating CONSTANS (CO) and FLOWERING LOCUS T (FT), and the col7 mutant had no obvious flowering phenotype under LD or SD conditions. So COL7 may have redundant functions with other CONSTANS-LIKEs (COLs) in controlling the flowering time of Arabidopsis. |
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| Keywords: | gene expression arabidopsis CONSTANS-LIKE 7 flowering |
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