| 中药提取物中总黄酮的分析研究 |
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| 引用本文： | 张静,余加进,戴建辉,朱荣岗,徐艳梅,王红斌.中药提取物中总黄酮的分析研究[J].云南民族大学学报(自然科学版),2010,19(6). |
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| 作者姓名： | 张静 余加进 戴建辉 朱荣岗 徐艳梅 王红斌 |
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| 作者单位： | 云南民族大学,化学与生物技术学院,云南,昆明,650031 |
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| 基金项目： | 云南省自然科学基金(2009CD100); 云南民族大学大学生创新实验计划(2009HSCX05) |
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| 摘 要： | 用NaNO2-Al(NO3)3-NaOH分光光度法测定了8种中药提取物中的总黄酮,考察了方法的回收率和精密度.实验表明:以芦丁为标准对照品、510 nm为测量波长,所得线性回归方程A=10.9942C+0.0015,相关系数R=0.9999.此方法稳定性好、操作简单快速、结果准确可靠,可作为中药提取物中总黄酮测定的分析方法.
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| 关 键 词： | 中药提取物 总黄酮 可见分光光度法 |
The Determination of Total Flavonoids from Chinese Herbal Extracts |
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| ZHANG Jing,YU Jia-jin,DAI Jian-hui,ZHU Rong-gang,XU Yan-mei,WANG Hong-bin.The Determination of Total Flavonoids from Chinese Herbal Extracts[J].Journal of Yunnan Nationalities University:Natural Sciences Edition,2010,19(6). |
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| Authors: | ZHANG Jing YU Jia-jin DAI Jian-hui ZHU Rong-gang XU Yan-mei WANG Hong-bin |
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| Affiliation: | ZHANG Jing,YU Jia-jin,DAI Jian-hui,ZHU Rong-gang,XU Yan-mei,WANG Hong-bin(School of Chemistry and Biotechnology,Yunnan University of Nationalities,Kunming 650031,China) |
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| Abstract: | The total flavonoids from eight Chinese herbal extracts were determined by NaNO2-Al(NO3)3-NaOH spectrophotometry and the recovery rate and precision were studied.The experiment showed that with rutin as the standard sample and NaNO2-Al(NO3)3-NaOH as the color reagent,the content of flavonoids was determined by VIS-spectrophotometry at the wavelength of 510 nm.The regression equation was A=10.942C+0.001 5,the correlation coefficient R=0.999 9.The results show that this method can be used to detect the total ... |
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| Keywords: | herbal extract total flavonoids VIS-spectrophotometry |
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