| 显脉旋覆花根中总黄酮提取方法的研究 |
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| 引用本文： | 张虹,李树伟,李春燕,袁寒,白红丽,郭俊明.显脉旋覆花根中总黄酮提取方法的研究[J].云南民族大学学报(自然科学版),2015(2):112-114. |
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| 作者姓名： | 张虹 李树伟 李春燕 袁寒 白红丽 郭俊明 |
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| 作者单位： | 红河学院云南省高校农作物优质高效栽培与安全控制重点实验室;云南民族大学民族药资源化学国家民委-教育部重点实验室 |
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| 基金项目： | 民族药资源化学国家民委-教育部重点实验室开放基金(MZY1405) |
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| 摘 要： | 以显脉旋覆花根为试验材料,用乙醇和甲醇为提取剂,采用浸提法分别对显脉旋覆花根中总黄酮进行提取,并用紫外分光光度法进行了测定.结果表明:乙醇70%,甲醇体积分数50%,浸提时间均为24 h时,总黄酮的提取效果较好,乙醇和甲醇提取的总黄酮得率分别为2.33%和1.84%,乙醇的提取效果优于甲醇,总黄酮测定方法平均回收率为99.89%,相对标准偏差RSD为0.89%,结果可靠.
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| 关 键 词： | 显脉旋覆花根 总黄酮 提取 |
Study on the extraction method of the total flavonoids in the roots of Inula nervosa |
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| ZHANG Hong;LI Shu-wei;LI Chun-yan;YUAN Han;BAI Hong-li;GUO Jun-ming.Study on the extraction method of the total flavonoids in the roots of Inula nervosa[J].Journal of Yunnan Nationalities University:Natural Sciences Edition,2015(2):112-114. |
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| Authors: | ZHANG Hong;LI Shu-wei;LI Chun-yan;YUAN Han;BAI Hong-li;GUO Jun-ming |
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| Affiliation: | ZHANG Hong;LI Shu-wei;LI Chun-yan;YUAN Han;BAI Hong-li;GUO Jun-ming;Key Laboratory of Crops with High-quality and Efficient Cultivation and Security Control of Yunnan Higher Education Institutions,Honghe University;Key Laboratory of Chemistry in Ethnic Medicinal Resources,State Ethnic Affairs Commission and Ministry of Education of China,Yunnan Minzu University; |
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| Abstract: | In this test,the roots of Inula nervosa were used as the test materials,and their total flavonoids were extracted by alcohol-and methanol-based cold-maceration. The content of the extracted total flavonoids was determined with UV spectrophotometer. The results were the following: while alcohol was 70% and methanol 50%,and the extracting time was 24 h,the extraction of the total flavonoids had a good effect. The extraction rate of alcohol and methanol was 2. 33% and 1. 84% respectively. Alcohol had a better effect than methanol. The average recovery of this method was 99. 89%,and the relative standard deviation( RSD) of the method was 0. 89%. Its results are reliable. |
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| Keywords: | roots of Inula nervosa total flavonoids extraction |
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