| 八角莲不同部位鬼臼毒素和4'-去甲鬼臼毒素含量的测定 |
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| 引用本文： | 马志刚,郑永雯,冯益青,王沛霖,杨保涛,邵娅婷,刘彦雄,曹秋娥.八角莲不同部位鬼臼毒素和4'-去甲鬼臼毒素含量的测定[J].云南民族大学学报(自然科学版),2014,23(5):317-319,330. |
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| 作者姓名： | 马志刚 郑永雯 冯益青 王沛霖 杨保涛 邵娅婷 刘彦雄 曹秋娥 |
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| 作者单位： | 云南大学化学科学与工程学院教育部自然资源药物化学重点实验室,云南昆明,650091 |
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| 基金项目： | 国家自然科学基金，国家大学生创新创业训练计划 |
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| 摘 要： | 利用反相高效液相色谱法建立了同时分离和测定4'-去甲鬼臼毒素和鬼臼毒素的方法.所选用的色谱柱为Waters Spherisorb ODS2(5μm,4.6 mm i.d×250 mm),流动相组成为甲醇-水(体积比为65∶35),检测波长为207 nm.在该色谱条件下,4'-去甲鬼臼毒素质量浓度在0.222 4556μg/mL、鬼臼毒素质量浓度在0.596 8556μg/mL、鬼臼毒素质量浓度在0.596 81 492μg/mL范围内线性关系良好,相关系数分别为0.999 8和0.999 9.该方法应用于中药八角莲的叶、茎、须和根中这2个组分的含量分析.
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| 关 键 词： | 八角莲 鬼臼毒素 4'-去甲鬼臼毒素 反相高效液相色谱法 |
Determination of podophyllotoxin and 4'-demethylpodophyllotoxin of Dysosma versipellis (Hance) M.Cheng by reversed-phase high-performance liquid chromatography |
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| MA Zhi-gang;ZHENG Yong-wen;FENG Yi-qing;WANG Pei-lin;YANG Bao-tao;SHAO Ya-ting;LIU Yan-xiong;CAO Qiu-e.Determination of podophyllotoxin and 4'-demethylpodophyllotoxin of Dysosma versipellis (Hance) M.Cheng by reversed-phase high-performance liquid chromatography[J].Journal of Yunnan Nationalities University:Natural Sciences Edition,2014,23(5):317-319,330. |
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| Authors: | MA Zhi-gang;ZHENG Yong-wen;FENG Yi-qing;WANG Pei-lin;YANG Bao-tao;SHAO Ya-ting;LIU Yan-xiong;CAO Qiu-e |
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| Affiliation: | MA Zhi-gang;ZHENG Yong-wen;FENG Yi-qing;WANG Pei-lin;YANG Bao-tao;SHAO Ya-ting;LIU Yan-xiong;CAO Qiu-e;Key Laboratory of Medicinal Chemistry for Natural Resources,Ministry of Education of China,School of Chemical Science and Technology,Yunnan University; |
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| Abstract: | A reversed- phase high- performance liquid chromatography( RP- HPLC) method has been developed for the determination of 4'- demethylpodophyllotoxin( DMEP) and podophyllotoxin( PPT). The results have indicated that DMEP and PPT could be separated by a Waters Spherisorb ODS2 column( 5 μm,4. 6 mm i. d. × 250mm) at the column temperature of 40 ℃,using methanol- water( 65∶ 35,V /V) as the mobile phase with the flow rate of 1. 0 mL /min and detective wavelength of 207 nm. Under the chromatographic conditions mentioned above,the linear ranges were found as 0. 222 4—556 μg /mL for DMEP and 0. 596 8—1 492 μg /mL for PPT,with the correlation coefficients of 0. 999 8 and 0. 999 9,respectively. The method could be used for the determination of DMEP and PPT in the Dysosm aversipellis( Hance) M. Cheng. |
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| Keywords: | Dysosma versipellis (Hance) M Cheng podophyllotoxin 4'-demethylpodophyllotoxin reversed-phase high-performance liquid chromatography |
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