| 水鳖蕨幼叶的离体快速繁殖 |
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| 引用本文： | 张光飞,苏文华.水鳖蕨幼叶的离体快速繁殖[J].云南大学学报(自然科学版),2002,24(3):234-236. |
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| 作者姓名： | 张光飞 苏文华 |
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| 作者单位： | 云南大学生态学与地植物学研究所,云南,昆明,650091 |
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| 基金项目： | 云南省自然科学基金资助项目 ( 94C0 0 5Q) |
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| 摘 要： | 通过组织培养技术可以从水鳖蕨Sinephropteris delavayi (Franch.)Mickel]的幼叶诱导出完整植株.研究结果表明,1/2MS+6-BA3.0mg/L(单位下同)+NAA0.5(蔗糖3%)适合于诱导绿色小球(GGB);适合于绿色小球的增殖培养基是1/2MS+6-BA2.0(蔗糖2%)+NAA0.2;适合于芽生长的培养基是1/2MS+NAA0.3;1/2MS(蔗糖2%不加任何外源激素)培养基适合于诱导生根,生根率可达100%.
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| 关 键 词： | 水鳖蕨 幼叶 组织培养 绿色小球 快速繁殖 |
| 文章编号： | 0258-7971(2002)03-0234-03 |
| 修稿时间： | 2001年5月22日 |
In vitro rapid propagation from young leaf of Sinephropteris delavayi ( Franch. ) Mickel |
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| ZHANG Guang-fei,SU Wen-hua.In vitro rapid propagation from young leaf of Sinephropteris delavayi ( Franch. ) Mickel[J].Journal of Yunnan University(Natural Sciences),2002,24(3):234-236. |
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| Authors: | ZHANG Guang-fei SU Wen-hua |
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| Affiliation: | Institute of Ecology &; Geobotany, Yunnan University, Kunming 650091, China |
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| Abstract: | Young leaves from Sinephropteris delavayi (Franch.)Mickel were used for tissue culture and rapid propagation.This experiment showed that the most proper medium for inducing green globular bodies (GGB) was 1/2MS+6-BA 3.0mg/L+NAA 0.5mg/L(sucrose 3%),the medium for multiplying GGB was 1/2MS+6-BA 2.0mg/L+NAA+0.2mg/L(sucrose 2%), the medium for GGB to shoot was 1/2MS+NAA0.3mg/L(sucrose 2% and no hormone).1/2MS (sucrose 2% and no hormone)was good for rooting,the rooting rate was 100%. |
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| Keywords: | Sinephropteris delavayi young leaf tissue culture green globular bodies (GGB) rapid propagation |
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