| 牛血清白蛋白亲和毛细管整体柱的制备及色氨酸对映体的分离 |
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| 引用本文： | 姚传义,贾静波,陈伟其.牛血清白蛋白亲和毛细管整体柱的制备及色氨酸对映体的分离[J].厦门大学学报(自然科学版),2010,49(1). |
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| 作者姓名： | 姚传义 贾静波 陈伟其 |
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| 作者单位： | 厦门大学化学化工学院,福建,厦门,361005 |
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| 基金项目： | 国家自然科学基金，福建省自然科学基金(E0610030)项目 |
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| 摘 要： | 利用亲和毛细管电色谱整体柱对色氨酸对映体进行了手性拆分研究.利用溶胶-凝胶法制备了壳聚糖/硅胶复合基质毛细管整体柱,然后采用戊二醛交联柱上键合牛血清白蛋白(BSA)得到亲和毛细管整体柱,以毛细管电色谱(CEC)模式分离色氨酸对映体,考察了缓冲液pH、浓度及有机修饰剂含量对分离过程的影响.在缓冲液的浓度为20 mmol/L,pH 7.5时,色氨酸对映体分离效果良好,分离度为2.44.
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| 关 键 词： | 壳聚糖 牛血清白蛋白 亲和整体柱 色氨酸对映体 |
Preparation of Bovine Serum Albumin Affinity Monolith for Chiral Separation of D,L-tryptophan by Capillary Electrochromatography |
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| YAO Chuan-yi,JIA Jing-bo,CHEN Wei-qi.Preparation of Bovine Serum Albumin Affinity Monolith for Chiral Separation of D,L-tryptophan by Capillary Electrochromatography[J].Journal of Xiamen University(Natural Science),2010,49(1). |
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| Authors: | YAO Chuan-yi JIA Jing-bo CHEN Wei-qi |
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| Affiliation: | YAO Chuan-yi,JIA Jing-bo,CHEN Wei-qi (College of Chemistry , Chemical Engineering,Xiamen University,Xiamen 361005,China) |
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| Abstract: | Enantioseparation of D,L-tryptophan was studied on an affinity monolithic column by capillary electrochromatography. A chitosan/silica hybrid monolithic column was prepared by the sol-gel method.Then the affinity stationary phase was synthesized by the in situ covalent immobilization of BSA on the monolithic column activated with glutaraldehyde.The monolith was used for chiral separation of D,L-tryptophan by capillary electrochromatography.The effects of pH,concentration of running buffer,and the content of... |
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| Keywords: | chitosan BSA affinity monolithic column tryptophan enantiomers |
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