萘对铜绿微囊藻和聚球藻生长及叶绿素荧光影响的比较

为探究萘对不同粒径蓝藻的胁迫效应,采用5组不同质量浓度萘(0、0.01 mg/L、0.1 mg/L、1 mg/L、10 mg/L)分别对聚球藻(Synechococcus sp.)和铜绿微囊藻(Microcystis aeruginosa,PCC7806)进行为期7 d的处理,分析不同浓度萘处理下,藻细胞浓度、叶绿素a含量及叶绿素荧光参数的变化,探究不同浓度萘处理对2种藻的生长及生理影响。结果表明:(a)整个处理周期内,聚球藻的生长被显著抑制,其叶绿素a含量减小了3.9%~40.4%;(b)聚球藻的潜在光合作用能力、光合作用速率均有所减弱,但其耐受强光的能力有所增强,说明聚球藻对萘具有较强的敏感性;(c)萘对铜绿微囊藻生长具有显著的促进作用,增强了其潜在光合作用能力,但抑制了其细胞内叶绿素a的合成。

Comparasion of the effects of naphthalene on the growth and chlorophyll fluorescence of Microcystis aeruginosa and Synechococcus sp.

To explore the stress effect of naphthalene on the cyanobacteria of different cell size, Microcystis aeruginosa and Synechococcus sp. were exposed to different concentration of naphthalene(0, 0. 01, 0. 1, 1, and 10 mg/L)for seven days. Changes of cell concentration, the content of chlorophyll a, and the chlorophyll fluorescence of algae in these groups were determined to investigate effects of naphthalene on the growth and physiological parameters of two species. Results showed that the growth of Synechococcus sp. was significantly inhibited by the naphthalene during the whole experimental. The chlorophyll a content of Synechococcus sp. declined by 3. 9%-40. 4%(compared with the control). The photosynthetic activity and the potential maximum photosynthetic rate of Synechococcus sp. both depressed due to the naphthalene stress, while its tolerance to strong light was improved. This indicates that Synechococcus sp. has a high sensitivity to naphthalene. On the contrary, the growth and the photosynthetic activity of Microcystis aeruginosa were significantly promoted. However, the synthesis of chlorophyll a in the cells of Microcystis aeruginosa was inhibited.