固定化双菌串联发酵生产1，3-丙二醇

以葡萄糖为底物，利用分别固定化的酵母Candida krusei ICM-Y-05和肺炎杆菌Klebsiella pneumoniae ZJU 5205串联发酵生产1,3-丙二醇。实验结果表明，由C.krusei发酵而得粗甘油只需经简单离心后即可被K.pneumoniae所利用。将C.krusei包埋于NaCS/PDMDAAC生物微胶囊中，并于气升式反应器中发酵，所得发酵液直接流入固定床反应器，同时向固定床反应器补充微量元素，发酵培养经NaCS/PDMDAAC生物微胶囊包埋的K.pneumoniae，产物即为1,3-丙二醇。三批次发酵实验结果表明，1,3-丙二醇/葡萄糖的最终摩尔转化率为0.295。

Production of 1,3-propanediol by co-culture of two immobilized microbes in series

Glucose has been converted to 1,3-propanediol using two bioreactors connected in series, the first being packed with immobilized Candida krusei ICM-Y-05 and the second with immobilized Klebsiella pneumoniae ZJU 5205. It was shown that crude glycerol formed by C. krusei could be consumed directly by K. pneumoniae. In subsequent experiments, C. krusei was encapsulated in sodium cellulose sulfate/polydimethyl(diallyl)ammonium chloride] (NaCS/PDMDAAC) capsules and fermentation carried out in an air-lift reactor. The glycerol produced was carried directly into the second reactor packed with encapsulated K. pneumoniae, with simultaneous continuous addition of a microelement solution, resulting in the formation of 1,3-propanediol. Three fermentation batches were carried out, with reproducible results. The observed yield of 1,3-propanediol was 0.295 mol per mol of glucose consumed.